product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ABCB1 / MDR1 / P Glycoprotein Antibody LS-B1448
catalog :
LS-B1448
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Kikuchi H, Maishi N, Annan D, Alam M, Dawood R, Sato M, et al. Chemotherapy-Induced IL8 Upregulates MDR1/ABCB1 in Tumor Blood Vessels and Results in Unfavorable Outcome. Cancer Res. 2020;80:2996-3008 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1448 image 1
Anti-MDR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
product information
AntibodyID :
40474
AntibodyName :
LS-B1448
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ABCB1 / MDR1 / P Glycoprotein Antibody LS-B1448
Specificity :
A BLAST analysis was used to suggest cross reactivity with ABCB1 protein from human, monkey, dog, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 255-280 of human ABCB1 protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Aliquot and store at -20°C or below for extended storage. Avoid freeze / thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.
IsotypeName :
IgG
Gene :
ABCB1 / MDR1 / P Glycoprotein
StandardGeneSymbol :
ABCB1
gene family :
Transporter
Subfamily :
ATP-binding cassette - ABCB/MDR
Reactivity :
Mouse, Dog, Rat, Human, Monkey
Usage :
ELISA (1:10000 - 1:50000), IHC, IHC-P (5 µg/ml), WB (1:200 - 1:2000)
ShortWebDescription :
P Glycoprotein antibody LS-B1448 is an unconjugated rabbit polyclonal antibody to P Glycoprotein (ABCB1 / MDR1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Tested in Western blot with recombinant proteins only. Immunohistochemistry: LS-B1448 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1448 was determined to be 5 ug/ml.
Synonyms :
ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.