product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ AML1 / RUNX1 Antibody (aa150-250) LS-B13948
catalog :
LS-B13948
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 3
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1h
  • western blot; mouse; loading ...; fig 1e
O Hare M, Amarnani D, Whitmore H, An M, Marino C, Ramos L, et al. Targeting Runt-Related Transcription Factor 1 Prevents Pulmonary Fibrosis and Reduces Expression of Severe Acute Respiratory Syndrome Coronavirus 2 Host Mediators. Am J Pathol. 2021;191:1193-1208 pubmed publisher
Whitmore H, Amarnani D, O Hare M, Delgado Tirado S, Gonzalez Buendia L, An M, et al. TNF-α signaling regulates RUNX1 function in endothelial cells. FASEB J. 2021;35:e21155 pubmed publisher
Delgado Tirado S, Amarnani D, Zhao G, Rossin E, Eliott D, Miller J, et al. Topical delivery of a small molecule RUNX1 transcription factor inhibitor for the treatment of proliferative vitreoretinopathy. Sci Rep. 2020;10:20554 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B13948 image 1
Western blot analysis of extracts of various cell lines, using RUNX1 antibody.
image 2 :
LifeSpan Biosciences LS-B13948 image 2
Immunofluorescence analysis of A549 cell using RUNX1 antibody. Blue: DAPI for nuclear staining.
image 3 :
LifeSpan Biosciences LS-B13948 image 3
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
product information
AntibodyID :
495769
AntibodyName :
LS-B13948
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ AML1 / RUNX1 Antibody (aa150-250) LS-B13948
Specificity :
Human AML1 / RUNX1
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa150-250
PresentationDesc :
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide of human RUNX1.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
AML1 / RUNX1
StandardGeneSymbol :
RUNX1
Reactivity :
Mouse, Rat, Human
Usage :
ICC (1:50 - 1:200), IF (1:50 - 1:200), IHC (1:50 - 1:100), IHC-P (1:100), IP (1:20 - 1:100), WB (1:500 - 1:2000)
ShortWebDescription :
RUNX1 antibody LS-B13948 is an unconjugated rabbit polyclonal antibody to RUNX1 (AML1) (aa150-250) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
The predicted MW is 20-28kDa/37kDa/48-51kDa, while the observed MW by Western blot was 65kDa.
Synonyms :
RUNX1, AMLCR1, Aml1 oncogene, AML1, AML1-EVI-1, CBF-alpha-2, CBFA2, Acute myeloid leukemia 1, EVI-1, PEBP2A2, PEA2-alpha B, AML1-EVI-1 fusion protein, Oncogene AML-1, PEBP2-alpha B, PEBP2aB
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.