product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NPHS1 / Nephrin Antibody LS-B1382
catalog :
LS-B1382
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Terai K, Jin D, Watase K, Imagawa A, Takai S. Mechanism of Albuminuria Reduction by Chymase Inhibition in Diabetic Mice. Int J Mol Sci. 2020;21: pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B1382 image 1
Anti-Nephrin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
image 2 :
LifeSpan Biosciences LS-B1382 image 2
Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 ug/ml.
image 3 :
LifeSpan Biosciences LS-B1382 image 3
Western blot of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
product information
AntibodyID :
40167
AntibodyName :
LS-B1382
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ NPHS1 / Nephrin Antibody LS-B1382
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
PBS containing 0.02% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A 14 AA synthetic peptide near the C-Terminus of human Nephrin. The immunogen is located within the last 50 AA of Nephrin.
PurificationDesc :
Peptide Column Affinity Chromatography
RecommendedStorageDesc :
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
IsotypeName :
IgG
Gene :
NPHS1 / Nephrin
BlockingPeptide :
LS-E29932
StandardGeneSymbol :
NPHS1
gene family :
Immunoglobulin
Reactivity :
Mouse, Rat, Human
Usage :
ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 µg/ml)
ShortWebDescription :
Nephrin antibody LS-B1382 is an unconjugated rabbit polyclonal antibody to Nephrin (NPHS1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Nephrin antibody can be used for detection of Nephrin by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 1 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Western Blot:
Synonyms :
NPHS1, Nephrin, NPHN, CNF
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.