IHC-plus™ HUWE1 / ARFBP1 Antibody LS-B1359 | LifeSpan Biosciences LS-B1359 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ HUWE1 / ARFBP1 Antibody LS-B1359

catalog :

LS-B1359

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

LifeSpan Biosciences product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:500; loading ...; fig s2a	Urbán N, van den Berg D, Forget A, Andersen J, Demmers J, Hunt C, et al. Return to quiescence of mouse neural stem cells by degradation of a proactivation protein. Science. 2016;353:292-5 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Chen L, Xu W, Yang M, Wang K, Chen Y, Huang X, et al. HUWE1 plays important role in mouse preimplantation embryo development and the dysregulation is associated with poor embryo development in humans. Sci Rep. 2016;6:37928 pubmed publisher
	He G, Chen Y, Liao H, Xu W, Zhang W, He G. Associations between Huwe1 and autophagy in rat cerebral neuron oxygen‑glucose deprivation and reperfusion injury. Mol Med Rep. 2020;22:5083-5094 pubmed publisher
	Guo Y, Li L, Xu T, Guo X, Wang C, Li Y, et al. HUWE1 mediates inflammasome activation and promotes host defense against bacterial infection. J Clin Invest. 2020;130:6301-6316 pubmed publisher
	He G, Xu W, Liao H, Jiang C, Li C, Zhang W. Silencing Huwe1 reduces apoptosis of cortical neurons exposed to oxygen-glucose deprivation and reperfusion. Neural Regen Res. 2019;14:1977-1985 pubmed publisher

image

image 1 :

Anti-HUWE1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 2 :

Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.

image 3 :

Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/ml.

product information

AntibodyID :

40144

AntibodyName :

LS-B1359

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ HUWE1 / ARFBP1 Antibody LS-B1359

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

PresentationDesc :

PBS containing 0.02% Sodium Azide

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

A 19 AA synthetic peptide near the C-Terminus of human ARF-BP1. The immunogen is located within aa 4250-4300 of ARF-BP1.

PurificationDesc :

Peptide Column Affinity Chromatography

RecommendedStorageDesc :

Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

IsotypeName :

IgG

Gene :

HUWE1 / ARFBP1

BlockingPeptide :

LS-E30348

StandardGeneSymbol :

HUWE1

Reactivity :

Mouse, Rat, Human

Usage :

ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (2 µg/ml)

ShortWebDescription :

ARFBP1 antibody LS-B1359 is an unconjugated rabbit polyclonal antibody to ARFBP1 (HUWE1) from human. It is reactive with human, mouse and rat. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 6 publications.

UsageText :

ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:

Synonyms :

HUWE1, ARF-binding protein 1, ARF-BP1, BJ-HCC-24 tumor antigen, Ib772, KIAA0312, LASU1, Mcl-1 ubiquitin ligase E3, HECTH9, MULE, HSPC272, KIAA1578, URE-B1, UREB1, URE-binding protein 1, HECT domain protein LASU1, Large structure of UREB1

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage