This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ MKI67 / Ki67 Antibody (aa1550-1700) LS-B13463
catalog :
LS-B13463
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
412665
AntibodyName :
LS-B13463
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
PathPlus™ MKI67 / Ki67 Antibody (aa1550-1700) LS-B13463
Specificity :
Human MKI67 / Ki67. Human MKI67 / Ki67
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa1550-1700
PresentationDesc :
PBS, 0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
A synthetic peptide made to an internal region of human Ki67 (within residues 1550-1700) [Swiss-Prot# P46013]
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
MKI67 / Ki67
StandardGeneSymbol :
MKI67
Reactivity :
Mouse, Rat, Pig, Human
Usage :
IF, IHC, IHC-Fr (1:50 - 1:300), IHC-P (5 µg/ml), WB
ShortWebDescription :
Ki67 antibody LS-B13463 is an unconjugated rabbit polyclonal antibody to Ki67 (MKI67) (aa1550-1700) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and WB.
UsageText :
This Ki67 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections and Immunocytochemistry/Immunofluorescence. There have been mixed results in Western blot. *ormalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval. This is probably the most critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein (immunofluorescence may give problems as the protein is nuclear).
Synonyms :
MKI67, Antigen KI-67, Ki67, KIA
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.