IHC-plus™ TUSC3 Antibody (Internal) LS-B13274 | LifeSpan Biosciences LS-B13274 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ TUSC3 Antibody (Internal) LS-B13274

catalog :

LS-B13274

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Sheng X, Xing S, Wang R, Chen K, Jia W. Low levels of tumor suppressor candidate 3 predict poor prognosis of patients with hepatocellular carcinoma. Onco Targets Ther. 2018;11:909-917 pubmed publisher

image

image 1 :

Western blot analysis of TUSC3 expression in SKBR3 (A); HeLa (B); HepG2 (C); MDAMB231 (D) whole cell lysates.

image 2 :

Immunohistochemical analysis of TUSC3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunofluorescent analysis of TUSC3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

product information

AntibodyID :

381057

AntibodyName :

LS-B13274

TargetSpecies :

Human

Host Species :

Rabbit

Product Name :

IHC-plus™ TUSC3 Antibody (Internal) LS-B13274

Specificity :

Recognizes endogenous levels of TUSC3 protein.

ClonalityDesc :

Polyclonal

AntibodyModification :

Unconjugated

AntigenModification :

Internal

PresentationDesc :

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

ImmunogenType :

Synthetic peptide - KLH conjugated

ImmunogenDesc :

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human TUSC3.

PurificationDesc :

Immunoaffinity purified

RecommendedStorageDesc :

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Gene :

TUSC3

BlockingPeptide :

LS-E41908

StandardGeneSymbol :

TUSC3

Reactivity :

Bovine, Human, Monkey

Usage :

ICC (1:100 - 1:500), IF, IHC, IHC-P (1:100), WB (1:500 - 1:1000)

ShortWebDescription :

TUSC3 antibody LS-B13274 is an unconjugated rabbit polyclonal antibody to TUSC3 (Internal) from human. It is reactive with human, bovine and monkey. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

Synonyms :

TUSC3, D8S1992, M33, MRT7, N33, OST3A, Tumor suppressor candidate 3, Protein N33, MRT22

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.