IHC-plus™ EIF4E Antibody LS-B12932 | LifeSpan Biosciences LS-B12932 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ EIF4E Antibody LS-B12932

catalog :

LS-B12932

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 1

Reference
Gao F, Li M, Yu X, Liu W, Zhou L, Li W. Licochalcone A inhibits EGFR signalling and translationally suppresses survivin expression in human cancer cells. J Cell Mol Med. 2021;25:813-826 pubmed publisher

image

image 1 :

Fluorescent image of HeLa cell stained with EIF4E Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). EIF4E immunoreactivity is localized to Cytoplasm significantly.

image 2 :

EIF4E Antibody western blot of HeLa cell line lysates (35 ug/lane). The EIF4E antibody detected the EIF4E protein (arrow).

image 3 :

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

product information

AntibodyID :

370241

AntibodyName :

LS-B12932

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ EIF4E Antibody LS-B12932

ClonalityDesc :

Monoclonal

AntibodyModification :

Unconjugated

PresentationDesc :

PBS, 0.09% Sodium Azide

PurificationDesc :

Protein G purified

RecommendedStorageDesc :

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

IsotypeName :

IgG1,k

Gene :

EIF4E

StandardGeneSymbol :

EIF4E

Reactivity :

Human

Usage :

IF (1:10 - 1:50), IHC, IHC-P (1:100), WB (1:100 - 1:500)

ShortWebDescription :

EIF4E antibody LS-B12932 is an unconjugated mouse monoclonal antibody to human EIF4E. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

Synonyms :

EIF4E, EIF-4E, EIF4E1, EIF4EL1, EIF4F, CBP, EIF-4F 25 kDa subunit, MRNA cap-binding protein

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.