This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
PathPlus™ CFTR Antibody (aa25-36) LS-B12931
catalog :
LS-B12931
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunocytochemistry, immunoprecipitation
product information
AntibodyID :
370240
AntibodyName :
LS-B12931
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
PathPlus™ CFTR Antibody (aa25-36) LS-B12931
Specificity :
Recognizes human CFTR, Mr 170kD. Two additional proteins were detected, Mr 52 and 38kD.
ClonalityDesc :
Monoclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa25-36
PresentationDesc :
PBS, pH 7.6, 0.09% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide RKGYRQRLELSD corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Horse, Rabbit, Pig, Armadillo (100%); Orangutan, Marmoset, Ferret, Sheep, Panda, Cat, Bovine, Dog, Bat, Turkey, Chicken, Lizard, Xenopus (92%); Mouse, Rat, Hamster, Elephant, Platypus (83%).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1,k
Gene :
CFTR
StandardGeneSymbol :
CFTR
gene family :
Transporter
Subfamily :
ATP-binding cassette - ABCC/MRP
Reactivity :
Human
Usage :
ICC, IP, WB (0.5 - 2 µg/ml)
ShortWebDescription :
CFTR antibody LS-B12931 is an unconjugated mouse monoclonal antibody to human CFTR (aa25-36). Validated for ICC, IP and WB.
UsageText :
WB: Detects CFTR in RIPA lysates from CFTR-transfected BHK cells. Reported to detect CFTR from a human T84 colon carcinoma epithelial cell lysate. CFTR-transfected BHK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with mouse anti-CFTR (0.5 ug/ml). Proteins were visualized using IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Note: DO NOT BOIL LYSATE. Instead incubate at 37°C for 30 minutes. ICC: Reported to immunostain CFTR in T84 cells. Note: CFTR runs as a diffuse protein on SDS-PAGE. IP: Immunoprecipitates CFTR from a T84 cell lysate.
Synonyms :
CFTR, ABC35, ABCC7, CF, DJ760C5.1, MRP7, CFTR/MRP, TNR-CFTR
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.