product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NEK2 Antibody LS-B129
catalog :
LS-B129
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 3
Reference
Ohta S, Ikemoto T, Wada Y, Saito Y, Yamada S, Imura S, et al. A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells. Sci Rep. 2019;9:18731 pubmed publisher
Wada Y, Ikemoto T, Morine Y, Imura S, Saito Y, Yamada S, et al. The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues. Sci Rep. 2019;9:13204 pubmed publisher
Ikemoto T, Feng R, Shimada M, Saito Y, Iwahashi S, Morine Y, et al. A New 2-Step Acceleration Protocol Using a Histone Deacetylase Inhibitor to Generate Insulin-Producing Cells From Adipose-Derived Mesenchymal Stem Cells. Pancreas. 2018;47:477-481 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B129 image 1
Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
product information
AntibodyID :
28050
AntibodyName :
LS-B129
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ NEK2 Antibody LS-B129
Specificity :
Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein.
PurificationDesc :
Affinity purified
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
NEK2
StandardGeneSymbol :
NEK2
gene family :
Protein Kinase
Subfamily :
NEK
Reactivity :
Human
Usage :
ELISA (1:2000 - 1:10000), IF, IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:2000)
ShortWebDescription :
NEK2 antibody LS-B129 is an unconjugated rabbit polyclonal antibody to human NEK2. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
UsageText :
Immunohistochemistry: LS-B129 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B129 was determined to be 2.5 ug/ml.
Synonyms :
NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-like protein kinase 1, NLK1, HSPK 21, NimA-related protein kinase 2
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.