This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ BRAF / B-Raf Antibody (aa10-22) LS-B12786
catalog :
LS-B12786
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
369669
AntibodyName :
LS-B12786
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ BRAF / B-Raf Antibody (aa10-22) LS-B12786
Specificity :
Recognizes B-Raf, Mr 95kD. Species cross-reactivity: mouse and rat.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa10-22
PresentationDesc :
PBS, pH 7.6, 0.25M sodium chloride, 0.09% sodium azide, 30% glycerol
ImmunogenType :
Synthetic peptide - KLH conjugated
ImmunogenDesc :
KLH-conjugated, synthetic peptide corresponding to aa10-22 (C-GGAEPGQALFNGD) of human B-Raf. The immunizing sequence has 12/13 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Marmoset, Mouse, Rat (92%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
BRAF / B-Raf
StandardGeneSymbol :
BRAF
gene family :
Protein Kinase
Subfamily :
Raf
Reactivity :
Human
Usage :
Func, IHC, IHC-P (1:100), WB
ShortWebDescription :
B-Raf antibody LS-B12786 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF) (aa10-22). Validated for Func, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Western Blot (MCF-7 and PC12 cells): 1:250-1:2000. MAP Kinase Activation and MBP Phosphorylation: 20-40ul Immunoprecipitated active B-Raf from NGF stimulated PC12 cell lysates, as demonstrated in a coupled phosphorylation assay that used the enzyme immunocomplex to phosphorylate and activate unactive MEK1 which then phosphorylated and activated MAP Kinase 2 (MAPK2), leading to phosphorylation of the substrate protein myelin basic protein (MBP) in vitro.
Synonyms :
BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.