product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ CAPN2 / Calpain 2 / M-Calpain Antibody (clone 1F4) LS-B12657
catalog :
LS-B12657
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1F4
reactivity :
human
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; human
Wang Y, Hall R, Lee M, Kamgar Parsi A, Bi X, Baudry M. The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation. Sci Rep. 2017;7:11771 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1e
Wang Y, Hersheson J, López D, Hammer M, Liu Y, Lee K, et al. Defects in the CAPN1 Gene Result in Alterations in Cerebellar Development and Cerebellar Ataxia in Mice and Humans. Cell Rep. 2016;16:79-91 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B12657 image 1
Western blot of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
image 2 :
LifeSpan Biosciences LS-B12657 image 2
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
product information
AntibodyID :
369065
AntibodyName :
LS-B12657
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ CAPN2 / Calpain 2 / M-Calpain Antibody (clone 1F4) LS-B12657
Specificity :
Human CAPN2 / Calpain 2 / M-Calpain
ClonalityDesc :
Monoclonal
CloneName :
1F4
AntibodyModification :
Unconjugated
PresentationDesc :
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
ImmunogenType :
Recombinant protein
ImmunogenDesc :
Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG2a
Gene :
CAPN2 / Calpain 2 / M-Calpain
StandardGeneSymbol :
CAPN2
gene family :
Protease
Subfamily :
Cysteine C2
Reactivity :
Mouse, Rat, Human, Monkey
Usage :
Flo (1:100), IHC, IHC-P (10 µg/ml), WB (1:500 - 1:2000)
ShortWebDescription :
M-Calpain antibody LS-B12657 is an unconjugated mouse monoclonal antibody to M-Calpain (CAPN2 / Calpain 2) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Synonyms :
CAPN2, Calpain 2, large subunit, Calpain ii, Calpain, large polypeptide L2, Calpain-2 catalytic subunit, Calpain 2, Calpain large polypeptide L2, Calpain-2 large subunit, CANP 2, Calpain M-type, CANP2, CANPL2, CANPml, M-type calpain, M-calpain, MCANP, Millimolar-calpain
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.