product summary
Loading...
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ KRT8 / CK8 / Cytokeratin 8 Antibody (aa401-450) LS-B12422
catalog :
LS-B12422
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s3a
Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
Joshi P, Waterhouse P, Kasaian K, Fang H, Gulyaeva O, Sul H, et al. PDGFRα+ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland. Nat Commun. 2019;10:1760 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B12422 image 1
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
image 2 :
LifeSpan Biosciences LS-B12422 image 2
Immunofluorescence analysis of HeLa cells, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.
image 3 :
LifeSpan Biosciences LS-B12422 image 3
Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using Keratin 8 Antibody. The lane on the right is blocked with the synthesized peptide.
product information
AntibodyID :
366086
AntibodyName :
LS-B12422
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ KRT8 / CK8 / Cytokeratin 8 Antibody (aa401-450) LS-B12422
Specificity :
Keratin 8 (Ab-431) Antibody detects endogenous levels of total Keratin 8 protein.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa401-450
PresentationDesc :
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
ImmunogenDesc :
Synthetic peptide from human Keratin 8. (AA Range: 401-450)
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Store at -20°C for up to 1 year.
IsotypeName :
IgG
Gene :
KRT8 / CK8 / Cytokeratin 8
StandardGeneSymbol :
KRT8
gene family :
Keratin
Reactivity :
Mouse, Rat, Human
Usage :
IF (1:100 - 1:500), IHC, IHC-P (1:50), Peptide-ELISA (1:1000), WB (1:500 - 1:1000)
ShortWebDescription :
Cytokeratin 8 antibody LS-B12422 is an unconjugated rabbit polyclonal antibody to Cytokeratin 8 (KRT8 / CK8) (aa401-450) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Western Blot: Predicted MW 53 kDa
Synonyms :
KRT8, CARD2, CK-8, CYK8, Cytokeratin-8, CK8, K2C8, K8, KO, Keratin-8, Type-II keratin Kb8, Cytokeratin 8, Keratin 8
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.