IHC-plus™ IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) LS-B123 | LifeSpan Biosciences LS-B123 product information

product summary

company name :

LifeSpan Biosciences

product type :

antibody

product name :

IHC-plus™ IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) LS-B123

catalog :

LS-B123

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

68C1039.2

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

LifeSpan Biosciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human immunohistochemistry; mouse	Kay A, Clark P, Maurer M, Ying S. Elevations in T-helper-2-initiating cytokines (interleukin-33, interleukin-25 and thymic stromal lymphopoietin) in lesional skin from chronic spontaneous ('idiopathic') urticaria. Br J Dermatol. 2015;172:1294-302 pubmed publisher
western blot; human	Terrier B, Bieche I, Maisonobe T, Laurendeau I, Rosenzwajg M, Kahn J, et al. Interleukin-25: a cytokine linking eosinophils and adaptive immunity in Churg-Strauss syndrome. Blood. 2010;116:4523-31 pubmed publisher
	Lam M, Hull L, Imrie A, Snidvongs K, Chin D, Pratt E, et al. Interleukin-25 and interleukin-33 as mediators of eosinophilic inflammation in chronic rhinosinusitis. Am J Rhinol Allergy. 2015;29:175-81 pubmed publisher

image

image 1 :

Anti-IL-25 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

product information

AntibodyID :

28044

AntibodyName :

LS-B123

TargetSpecies :

Human

Host Species :

Mouse

Product Name :

IHC-plus™ IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) LS-B123

Specificity :

A synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence, was used as immunogen.

ClonalityDesc :

Monoclonal

CloneName :

68C1039.2

AntibodyModification :

Unconjugated

AntigenModification :

aa115-132

PresentationDesc :

PBS with 0.05% sodium azide.

ImmunogenType :

Synthetic peptide

ImmunogenDesc :

Synthetic peptide from human IL25 / IL-25.

PurificationDesc :

Protein G purified

RecommendedStorageDesc :

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IsotypeName :

IgG1

Gene :

IL25 / IL17E

StandardGeneSymbol :

IL25

gene family :

Interleukin

Reactivity :

Mouse, Human

Usage :

IHC, IHC-P (10 µg/ml), WB (2 - 4 µg/ml)

ShortWebDescription :

IL17E antibody LS-B123 is an unconjugated mouse monoclonal antibody to IL17E (IL25) (aa115-132) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

UsageText :

Immunohistochemistry: LS-B123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody , followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B123 was determined to be 10 ug/ml.

Synonyms :

IL25, Interleukin 17E, Interleukin 25, Interleukin-25, IL17E, IL-17E, IL-25, Interleukin-17E

SalesRegion :

Worldwide

more info or order :

LifeSpan Biosciences product webpage

company information

LifeSpan Biosciences

2401 Fourth Avenue, Suite 900
Seattle, WA 98121

CustomerSupport@lsbio.com

https://www.lsbio.com

1-206-464-1554

headquarters: USA

Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.