This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SMAD7 Antibody (aa36-50) LS-B120
catalog :
LS-B120
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
28041
AntibodyName :
LS-B120
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SMAD7 Antibody (aa36-50) LS-B120
Specificity :
Two synthetic peptides corresponding to aa 12-18 (RLWRSRAPGGEDEEEGAG) and amino acids 36-50 (ELRGEGATDSRAHGA) of human SMAD7; GenBank Accession no. AAB81354.1. This sequence is 100% or highly conserved between human and multiple species.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa36-50
PresentationDesc :
PBS, 0.05% Sodium Azide, 0.05% BSA
ImmunogenDesc :
Amino acids 12-29 (RLWRSRAPGGEDEEEGAG) and 36-50 (ELRGEGATDSRAHGA) of human SMAD7. This sequence is 100% or highly conserved between human and multiple species. Percent identity by BLAST analysis: Human, Monkey, Bovine, Dog, Bat, Panda (100%); Mouse, Rat, Rabbit, Pig, Platypus (94%).
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
SMAD7
StandardGeneSymbol :
SMAD7
Reactivity :
Dog, Bovine, Bat, Human, Monkey
Usage :
IHC, IHC-P (10 µg/ml), WB (1:500 - 1:1000)
ShortWebDescription :
SMAD7 antibody LS-B120 is an unconjugated rabbit polyclonal antibody to SMAD7 (aa36-50) from human. It is reactive with human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B120 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B120 was determined to be 10 ug/ml.
Synonyms :
SMAD7, CRCS3, HSMAD7, MAD homolog 7, SMAD 7, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, MAD homolog 8, SMAD family member 7
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.