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product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ POU5F1 / OCT4 Antibody (aa114-163) LS-B1194
catalog :
LS-B1194
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
34188
AntibodyName :
LS-B1194
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ POU5F1 / OCT4 Antibody (aa114-163) LS-B1194
Specificity :
Human POU5F1 / OCT4
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa114-163
PresentationDesc :
PBS, 0.09% sodium azide, 2% sucrose
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%); Marmoset (92%); Dog (91%); Bovine, Pig, Guinea pig (85%); Horse (84%); Cat (83%).
PurificationDesc :
Protein A/G purified
RecommendedStorageDesc :
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Gene :
POU5F1 / OCT4
BlockingPeptide :
LS-E10933
StandardGeneSymbol :
POU5F1
Reactivity :
Rabbit, Goat, Pig, Human
Usage :
IHC, IHC-P (5 µg/ml), WB (1.25 µg/ml)
ShortWebDescription :
OCT4 antibody LS-B1194 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) (aa114-163) from human. It is reactive with human, goat, pig and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B1194 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1194 was determined to be 5 ug/ml.
Synonyms :
POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3, OCT3/4
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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