product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ EGF Antibody (aa106-119) LS-B11905
catalog :
LS-B11905
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human, domestic rabbit
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; domestic rabbit; fig 3
  • western blot; human
Li Y, Zhang J, Xu Y, Han Y, Jiang B, Huang L, et al. The Histopathological Investigation of Red and Blue Light Emitting Diode on Treating Skin Wounds in Japanese Big-Ear White Rabbit. PLoS ONE. 2016;11:e0157898 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B11905 image 1
EGF antibody (1 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
image 2 :
LifeSpan Biosciences LS-B11905 image 2
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
product information
AntibodyID :
353571
AntibodyName :
LS-B11905
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ EGF Antibody (aa106-119) LS-B11905
Specificity :
Human EGF. This antibody is expected to recognize all reported isoforms (NP_001954.2; NP_001171601.1; NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa106-119
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence SRQERVCNIEKNVS, from the internal region (near the N-Terminus) of the protein sequence according to NP_001954.2NP_001171601.1NP_001171602.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
EGF
BlockingPeptide :
LS-E46822
StandardGeneSymbol :
EGF
Reactivity :
Rabbit, Human, Monkey
Usage :
IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
ShortWebDescription :
EGF antibody LS-B11905 is an unconjugated goat polyclonal antibody to EGF (aa106-119) from human. It is reactive with human, monkey and rabbit. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 160kD double band observed in Human Prostate lysates (calculated MW of 134kD according to NP_001954.2). The two bands represent glycosylated forms of the immature protein. Recommended concentration: 1-3 ug/ml.
Synonyms :
EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.