This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ AKT1 + AKT2 + AKT3 Antibody (phospho-Ser473) LS-B1183
catalog :
LS-B1183
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
product information
AntibodyID :
34177
AntibodyName :
LS-B1183
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ AKT1 + AKT2 + AKT3 Antibody (phospho-Ser473) LS-B1183
Specificity :
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKTpS473. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
pSer473
PresentationDesc :
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Rabbit Anti-AKTpS473 Antibody was prepared by repeated immunizations in rabbits with a synthetic peptide corresponding to a C-terminus region near phospho Serine 473 of the human, mouse, rat and chicken AKT proteins conjugated to KLH.
PurificationDesc :
Affinity chromatography
RecommendedStorageDesc :
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
AKT1 + AKT2 + AKT3
Reactivity :
Mouse, Rat, Human
Usage :
ELISA (1:15000 - 1:60000), IF, IHC, IHC-P (5 µg/ml), WB (1:200 - 1:1000)
ShortWebDescription :
AKT1 + AKT2 + AKT3 antibody LS-B1183 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 (pSer473) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B1183 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1183 was determined to be 5 ug/ml.
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.