This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SELPLG / PSGL-1 / CD162 Antibody (clone KPL-1) LS-B11572
catalog :
LS-B11572
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
KPL-1
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
341105
AntibodyName :
LS-B11572
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ SELPLG / PSGL-1 / CD162 Antibody (clone KPL-1) LS-B11572
Specificity :
P-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}
ClonalityDesc :
Monoclonal
CloneName :
KPL-1
AntibodyModification :
Unconjugated
PresentationDesc :
0.02 M PB, pH 7.6, 0.25 M NaCl, 0.1% Sodium Azide
ImmunogenDesc :
Mouse murine 300.19 pre B-cell line expressing full length human PSGL-1
PurificationDesc :
Purified
RecommendedStorageDesc :
Store at 4°C. Store undiluted.
IsotypeName :
IgG1
Gene :
SELPLG / PSGL-1 / CD162
StandardGeneSymbol :
SELPLG
Reactivity :
Human
NonReactiveSpecies :
Bovine, Rat, Pig, Equine
Usage :
Flo, IHC, IHC-Fr, IHC-P (10 µg/ml), IP, WB
ShortWebDescription :
CD162 antibody LS-B11572 is an unconjugated mouse monoclonal antibody to human CD162 (SELPLG / PSGL-1). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Western blot of HL60 whole cell lysates prepared with 1% Triton X-100 in 150 mM NaCl, 10 mM Tris-HCl pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1 mM aprotinin, PMSF, leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000. FACS {Snapp, KR et al 1998}. Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein). Functional blocking of P-selectin {Snapp KR et al 1998}. Immunohistochemistry: Fresh frozen, acetone fixed sections. Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 10ug/ml. Optimal working dilutions must be determined by the end user.
Synonyms :
SELPLG, CD162 antigen, CLA, CD162, PSGL1, PSGL-1, Selectin P ligand
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.
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