product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ LIN28A / LIN28 Antibody (aa108-138) LS-B11566
catalog :
LS-B11566
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1
Reference
Amen A, Ruiz Garzon C, Shi J, Subramanian M, Pham D, Meffert M. A Rapid Induction Mechanism for Lin28a in Trophic Responses. Mol Cell. 2017;65:490-503.e7 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-B11566 image 1
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
image 2 :
LifeSpan Biosciences LS-B11566 image 2
Fluorescent confocal image of SY5Y cells stained LIN28A antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated LIN28A primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
image 3 :
LifeSpan Biosciences LS-B11566 image 3
LIN28A Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The LIN28A antibody detected the LIN28A protein (arrow).
product information
AntibodyID :
341097
AntibodyName :
LS-B11566
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ LIN28A / LIN28 Antibody (aa108-138) LS-B11566
Specificity :
This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the Central region of human LIN28A.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa108-138
PresentationDesc :
PBS, 0.09% Sodium Azide
PurificationDesc :
Ammonium sulfate precipitation
RecommendedStorageDesc :
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Gene :
LIN28A / LIN28
BlockingPeptide :
LS-E833
StandardGeneSymbol :
LIN28A
Reactivity :
Mouse, Human
Usage :
Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC, IHC-P (1:100), WB (1:1000)
ShortWebDescription :
LIN28 antibody LS-B11566 is an unconjugated rabbit polyclonal antibody to LIN28 (LIN28A) (aa108-138) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Synonyms :
LIN28A, CSDD1, Lin-28 homolog (C. elegans), LIN-28, Lin-28A, LIN28, Protein lin-28 homolog A, Lin-28 homolog A (C. elegans), ZCCHC1, RNA-binding protein LIN-28
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.