product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ NR4A1 / NUR77 Antibody (aa251-266) LS-B114
catalog :
LS-B114
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human
Wu H, Bi J, Peng Y, Huo L, Yu X, Yang Z, et al. Nuclear receptor NR4A1 is a tumor suppressor down-regulated in triple-negative breast cancer. Oncotarget. 2017;8:54364-54377 pubmed publisher
product information
AntibodyID :
28035
AntibodyName :
LS-B114
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ NR4A1 / NUR77 Antibody (aa251-266) LS-B114
Specificity :
A synthetic peptide corresponding to aa 251-266 of human Nak1.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa251-266
PresentationDesc :
PBS, 0.05% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic peptide from human NR4A1 / NUR77.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG
Gene :
NR4A1 / NUR77
StandardGeneSymbol :
NR4A1
gene family :
NHR
Subfamily :
NR4 Nerve growth factor-like
Reactivity :
Mouse, Rat, Human
Usage :
ChrIP (1:10 - 1:500), ICC, IF, IHC, IHC-P (20 - 40 µg/ml), IP (1:10 - 1:500), WB (1:500 - 1:1000)
ShortWebDescription :
NUR77 antibody LS-B114 is an unconjugated rabbit polyclonal antibody to NUR77 (NR4A1) (aa251-266) from human. It is reactive with human, mouse and rat. Validated for ChrIP, ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
UsageText :
Immunohistochemistry: LS-B114 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B114 was determined to be 20-40 ug/ml.
Synonyms :
NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, Orphan nuclear receptor HMR
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.