This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa329-530, clone ESEE122) LS-B109
catalog :
LS-B109
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ESEE122

The same clone is also sold as:
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
product information
AntibodyID :
28030
AntibodyName :
LS-B109
TargetSpecies :
Human
Host Species :
Mouse
Product Name :
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa329-530, clone ESEE122) LS-B109
Specificity :
HIF-1 alpha.
ClonalityDesc :
Monoclonal
CloneName :
ESEE122
AntibodyModification :
Unconjugated
AntigenModification :
aa329-530
PresentationDesc :
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
ImmunogenType :
Purified protein
ImmunogenDesc :
Human HIF-1 alpha amino acids 329-530.
PurificationDesc :
Protein G purified
RecommendedStorageDesc :
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
IsotypeName :
IgG1
Gene :
HIF1A / HIF1 Alpha
StandardGeneSymbol :
HIF1A
Reactivity :
Mouse, Dog, Bovine, Rat, Human
Usage :
ICC, IF (8 - 12 µg/ml), IHC, IHC-Fr (1:100 - 1:5000), IHC-P (1.25 µg/ml), IP (1:10 - 1:1000), WB (1:500 - 1:1000)
ShortWebDescription :
HIF1 Alpha antibody LS-B109 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) (aa329-530) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: LS-B109 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B109 was determined to be 1.25 ug/ml.
Synonyms :
HIF1A, ARNT-interacting protein, ARNT interacting protein, BHLHe78, HIF1 Alpha, Hypoxia-inducible factor1alpha, HIF-1A, HIF1, MOP1, Member of PAS superfamily 1, PASD8, HIF-1-alpha, HIF-1alpha, HIF1-ALPHA, Member of PAS protein 1
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.