This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa339-351) LS-B10396
catalog :
LS-B10396
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
295061
AntibodyName :
LS-B10396
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa339-351) LS-B10396
Specificity :
Human MAPKAPK2 / MK2. This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa339-351
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-SRVLKEDKERWED, from the internal region (near C-Terminus) of the protein sequence according to NP_004750.1NP_116584.2.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
MAPKAPK2 / MAPKAP Kinase 2
BlockingPeptide :
LS-E28035
StandardGeneSymbol :
MAPKAPK2
gene family :
Protein Kinase
Subfamily :
MAPKAPK
Reactivity :
Rabbit, Mouse, Dog, Guinea pig, Bovine, Hamster, Pig, Horse, Human
Usage :
IHC, IHC-P (5 µg/ml), IP (1.5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 2 µg/ml)
ShortWebDescription :
MAPKAP Kinase 2 antibody LS-B10396 is an unconjugated goat polyclonal antibody to MAPKAP Kinase 2 (MAPKAPK2) (aa339-351) from human. It is reactive with human, mouse, bovine and other species. Validated for IHC, IP, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 60kD band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kD band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kD according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec;66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2 ug/ml. Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced with MK2 or empty vector were lysed from confluent plates and used for IP with 1.5 ug EB11929 or EB11930. Western blots of the IP were labeled with rabbit anti-MK2 (CST). An approx 45kD of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Synonyms :
MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.