This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MAPK6 / ERK3 Antibody (aa683-694) LS-B10395
catalog :
LS-B10395
clonality :
polyclonal
host :
domestic goat
conjugate :
nonconjugated
reactivity :
human
application :
western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
product information
AntibodyID :
295060
AntibodyName :
LS-B10395
TargetSpecies :
Human
Host Species :
Goat
Product Name :
IHC-plus™ MAPK6 / ERK3 Antibody (aa683-694) LS-B10395
Specificity :
Human MAPK6 / ERK3. Reported variants represent identical protein (NP_056621.4; NP_081694.1).
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
aa683-694
PresentationDesc :
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
ImmunogenDesc :
Peptide with sequence C-HSPVGSPLKSIQ, from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.
PurificationDesc :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
RecommendedStorageDesc :
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Gene :
MAPK6 / ERK3
BlockingPeptide :
LS-E28032
StandardGeneSymbol :
MAPK6
gene family :
Protein Kinase
Subfamily :
MAPK
Reactivity :
Chicken, Bovine, Rabbit, Mouse, Dog, Hamster, Pig, Horse, Human
Usage :
IHC, IHC-P (10 µg/ml), IP (1.5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 1 µg/ml)
ShortWebDescription :
ERK3 antibody LS-B10395 is an unconjugated goat polyclonal antibody to ERK3 (MAPK6) (aa683-694) from human. It is reactive with human, mouse, bovine and other species. Validated for IHC, IP, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
UsageText :
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1 ug/ml. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 ug EB11926. Western blots of the IP were labeled with rabbit anti-ERK3 serum (CST) or with mouse anti-MK5 (SC). An approx 100kD of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Synonyms :
MAPK6, ERK-3, MAP kinase 6, MAPK 6, p97-MAPK, PRKM6, MAP kinase isoform p97, ERK3, HsT17250, p97MAPK
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.