product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ ITGA4 / VLA-4 / CD49d Antibody (Internal) LS-A9385
catalog :
LS-A9385
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 1
Reference
Pulkka O, Mpindi J, Tynninen O, Nilsson B, Kallioniemi O, Sihto H, et al. Clinical relevance of integrin alpha 4 in gastrointestinal stromal tumours. J Cell Mol Med. 2018;22:2220-2230 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-A9385 image 1
Anti-VLA-4 / CD49D antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.
product information
AntibodyID :
159038
AntibodyName :
LS-A9385
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ ITGA4 / VLA-4 / CD49d Antibody (Internal) LS-A9385
Specificity :
Human ITGA4 / VLA-4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
Internal
PresentationDesc :
PBS, 0.1% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic 14 amino acid peptide from internal region of human ITGA4 / VLA-4. Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (100%), Monkey (100%), Marmoset (100%), Mouse (100%), Rat (100%), Elephant (93%), Panda (93%), Dog (86%), Bat (86%), Bovine (86%), Rabbit (86%), Horse (86%), Pig (86%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Gene :
ITGA4 / VLA-4 / CD49d
BlockingPeptide :
LS-E29625
StandardGeneSymbol :
ITGA4
gene family :
Integrin
Reactivity :
Mouse, Rat, Human, Monkey
Usage :
IHC, IHC-P (10 - 20 µg/ml)
ShortWebDescription :
CD49d antibody LS-A9385 is an unconjugated rabbit polyclonal antibody to CD49d (ITGA4 / VLA-4) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
UsageText :
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 - 20 ug/ml.
Synonyms :
ITGA4, 269C wild type, CD49D, IA4, Integrin alpha-4, Integrin alpha 4, Integrin alpha-4 subunit, Integrin alpha-IV, VLA-4, VLA-4 subunit alpha, CD49d antigen
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.