product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ SMO / Smoothened Antibody (N-Terminus) LS-A2666
catalog :
LS-A2666
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
LifeSpan Biosciences product webpage
citations: 5
Reference
Jamal M, Nunes A, Vaziri N, Ramchandran R, Bacallao R, Nauli A, et al. Rapamycin treatment correlates changes in primary cilia expression with cell cycle regulation in epithelial cells. Biochem Pharmacol. 2020;178:114056 pubmed publisher
Ferent J, Giguère F, Jolicoeur C, Morin S, Michaud J, Makihara S, et al. Boc Acts via Numb as a Shh-Dependent Endocytic Platform for Ptch1 Internalization and Shh-Mediated Axon Guidance. Neuron. 2019;102:1157-1171.e5 pubmed publisher
Hehemann M, Kalmanek E, Choe S, Dynda D, Hu W, Quek M, et al. Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence. Neurourol Urodyn. 2018;37:2551-2559 pubmed publisher
Toomer K, Fulmer D, Guo L, Drohan A, Peterson N, Swanson P, et al. A role for primary cilia in aortic valve development and disease. Dev Dyn. 2017;246:625-634 pubmed publisher
Dores C, Alpaugh W, Su L, Biernaskie J, Dobrinski I. Primary cilia on porcine testicular somatic cells and their role in hedgehog signaling and tubular morphogenesis in vitro. Cell Tissue Res. 2017;368:215-223 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-A2666 image 1
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
product information
AntibodyID :
161852
AntibodyName :
LS-A2666
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ SMO / Smoothened Antibody (N-Terminus) LS-A2666
Specificity :
Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, 0.1% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic 15 amino acid peptide from N-Terminus extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Gene :
SMO / Smoothened
BlockingPeptide :
LS-E28591
StandardGeneSymbol :
SMO
gene family :
GPCR
Subfamily :
Frizzled
Reactivity :
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
Usage :
ICC, IF, IHC, IHC-P (10 µg/ml)
ShortWebDescription :
Smoothened antibody LS-A2666 is an unconjugated rabbit polyclonal antibody to Smoothened (SMO) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 8 publications.
UsageText :
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Synonyms :
SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog
SalesRegion :
Worldwide
more info or order :
LifeSpan Biosciences product webpage
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.