product summary
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company name :
LifeSpan Biosciences
product type :
antibody
product name :
IHC-plus™ MMP13 Antibody (N-Terminus) LS-A1552
catalog :
LS-A1552
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
immunohistochemistry, immunohistochemistry - paraffin section
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; fig 3g
Abdelgawad M, Delaisse J, Hinge M, Jensen P, Alnaimi R, Rolighed L, et al. Early reversal cells in adult human bone remodeling: osteoblastic nature, catabolic functions and interactions with osteoclasts. Histochem Cell Biol. 2016;145:603-15 pubmed publisher
image
image 1 :
LifeSpan Biosciences LS-A1552 image 1
Anti-MMP13 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
product information
AntibodyID :
48139
AntibodyName :
LS-A1552
TargetSpecies :
Human
Host Species :
Rabbit
Product Name :
IHC-plus™ MMP13 Antibody (N-Terminus) LS-A1552
Specificity :
Human MMP13. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
ClonalityDesc :
Polyclonal
AntibodyModification :
Unconjugated
AntigenModification :
N-Terminus
PresentationDesc :
PBS, 0.1% Sodium Azide
ImmunogenType :
Synthetic peptide
ImmunogenDesc :
Synthetic 18 amino acid peptide from N-Terminus of human MMP13. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon, Monkey (94%); Marmoset (83%).
PurificationDesc :
Immunoaffinity purified
RecommendedStorageDesc :
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Gene :
MMP13
BlockingPeptide :
LS-E28323
StandardGeneSymbol :
MMP13
gene family :
Protease
Subfamily :
Metallopeptidase M10A
Reactivity :
Human
Usage :
IHC, IHC-P (17 µg/ml)
ShortWebDescription :
MMP13 antibody LS-A1552 is an unconjugated rabbit polyclonal antibody to human MMP13 (N-Terminus). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
UsageText :
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 17 ug/ml.
Synonyms :
MMP13, Collagenase 3, Matrix metalloproteinase-13, Interstitial collagenase, MANDP1, MMP-13, CLG3
SalesRegion :
Worldwide
company information
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
CustomerSupport@lsbio.com
https://www.lsbio.com
1-206-464-1554
headquarters: USA
Since 1995, LifeSpan has been the industry leader in molecular pathology, specializing in the localization of proteins in normal and diseased tissues, both human and non-human. We offer more than 74,000 antibodies, custom designed immunohistochemistry (IHC) studies, immediately available human tissue IHC profiles for more than 500 proteins, and histology and pathology services. Our bank of 2 million specimens is available to support our customers' contract research studies and contains frozen and formalin-fixed (FFPE) normal and diseased tissues. Our contract services are comprehensive; they include study design, antibody sourcing and characterization, tissue sourcing and validation, immunolabeling, trouble shooting, and interpretation of the results by a LifeSpan pathologist.