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> LifeSpan Biosciences SMG7 antibody
SMG7 Antibody (Internal) LS-C353204
LifeSpan Biosciences
catalog: LS-C353204
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunoprecipitation
,
immunohistochemistry - paraffin section
,
western blot knockout validation
citations: 1
Western blot analysis of SMG7 expression in Jurkat (A); A431 (B); HeLa (C) whole cell lysates.
Immunohistochemical analysis of SMG7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of SMG7 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
SMG7 Antibody (aa521-570) LS-C119181
LifeSpan Biosciences
catalog: LS-C119181
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunocytochemistry
Immunofluorescence analysis of HepG2 cells, using SMG7 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa, Jurkat, 293, and HUVEC cells, using SMG7 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
SMG7 Antibody LS-C155648
LifeSpan Biosciences
catalog: LS-C155648
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE SMG7 antibody diluted at 1:1000
IHC of paraffin-embedded HeLa xenograft, using SMG7 antibody antibody at 1:500 dilution.
quantity:
price:
to the supplier
SMG7 Antibody (Lys548) LS-C191987
LifeSpan Biosciences
catalog: LS-C191987
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
quantity:
price:
to the supplier
SMG7 Antibody (aa1087-1137) LS-C288924
LifeSpan Biosciences
catalog: LS-C288924
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunoprecipitation
quantity:
price:
to the supplier
SMG7 Antibody (aa896-930) LS-C344489
LifeSpan Biosciences
catalog: LS-C344489
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Western blot of lysates from HT-29, Jurkat, MCF-7 cell line (from left to right) with SMG7 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
quantity:
price:
to the supplier
SMG7 Antibody (490-570 aa, Internal) LS-C386102
LifeSpan Biosciences
catalog: LS-C386102
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
,
immunocytochemistry
Western blot of SMG7 antibody
quantity:
price:
to the supplier