IHC-plus™ TBC1D4 / AS160 Antibody (aa825-837) LS-B2764
LifeSpan Biosciences
catalog: LS-B2764
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody (0.1 ug/ml) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TBC1D4 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
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TBC1D4 / AS160 Antibody (phospho-Thr642) LS-C34921
LifeSpan Biosciences
catalog: LS-C34921
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA
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TBC1D4 / AS160 Antibody (aa1178-1189) LS-C63888
LifeSpan Biosciences
catalog: LS-C63888
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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TBC1D4 / AS160 Antibody (aa611-660) LS-C117628
LifeSpan Biosciences
catalog: LS-C117628
domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry

Immunofluorescence analysis of HeLa cells, using AS160 Antibody. The picture on the right is blocked with the synthesized peptide.
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TBC1D4 / AS160 Antibody (phospho-Ser666) LS-C125034
LifeSpan Biosciences
catalog: LS-C125034
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot
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TBC1D4 / AS160 Antibody (phospho-Thr642) LS-C126663
LifeSpan Biosciences
catalog: LS-C126663
domestic rabbit polyclonal
reactivity: human
application: western blot
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TBC1D4 / AS160 Antibody LS-C147066
LifeSpan Biosciences
catalog: LS-C147066
domestic rabbit polyclonal
reactivity: human
application: western blot
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TBC1D4 / AS160 Antibody (aa172-201) LS-C161734
LifeSpan Biosciences
catalog: LS-C161734
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of TBC1D4 Antibody in MDA-MB231 cell line lysates (35 ug/lane). TBC1D4 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TBC1D4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TBC1D4 Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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TBC1D4 / AS160 Antibody (clone OTI5E6) LS-C172853
LifeSpan Biosciences
catalog: LS-C172853
mouse monoclonal (OTI5E6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.

Anti-TBC1D4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.
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TBC1D4 / AS160 Antibody (clone OTI5A6) LS-C172861
LifeSpan Biosciences
catalog: LS-C172861
mouse monoclonal (OTI5A6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.

Anti-TBC1D4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D4 antibody, and then analyzed by flow cytometry.
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TBC1D4 / AS160 Antibody (phospho-Thr642) LS-C198645
LifeSpan Biosciences
catalog: LS-C198645
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.
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TBC1D4 / AS160 Antibody (phospho-Thr642) LS-C209365
LifeSpan Biosciences
catalog: LS-C209365
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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TBC1D4 / AS160 Antibody (aa172-201, APC) LS-C283394
LifeSpan Biosciences
catalog: LS-C283394
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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TBC1D4 / AS160 Antibody (aa172-201, AP) LS-C283395
LifeSpan Biosciences
catalog: LS-C283395
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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TBC1D4 / AS160 Antibody (aa172-201, Biotin) LS-C283396
LifeSpan Biosciences
catalog: LS-C283396
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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TBC1D4 / AS160 Antibody (aa172-201, FITC) LS-C283397
LifeSpan Biosciences
catalog: LS-C283397
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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TBC1D4 / AS160 Antibody (aa172-201, Azide-free, HRP) LS-C283398
LifeSpan Biosciences
catalog: LS-C283398
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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TBC1D4 / AS160 Antibody (aa172-201, PE) LS-C283399
LifeSpan Biosciences
catalog: LS-C283399
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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TBC1D4 / AS160 Antibody (aa200-250) LS-C290385
LifeSpan Biosciences
catalog: LS-C290385
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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TBC1D4 / AS160 Antibody (aa1-50) LS-C290387
LifeSpan Biosciences
catalog: LS-C290387
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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TBC1D4 / AS160 Antibody (aa1248-1298) LS-C290388
LifeSpan Biosciences
catalog: LS-C290388
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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TBC1D4 / AS160 Antibody (aa172-201) LS-C328848
LifeSpan Biosciences
catalog: LS-C328848
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ TBC1D4 / AS160 Antibody (aa1155-1204) LS-B11556
LifeSpan Biosciences
catalog: LS-B11556
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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TBC1D4 / AS160 Antibody (phospho-Thr642) LS-C342101
LifeSpan Biosciences
catalog: LS-C342101
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
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TBC1D4 / AS160 Antibody (phospho-Thr642) LS-C353214
LifeSpan Biosciences
catalog: LS-C353214
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of AS160 (pT642) expression in LO2 insulin-treated (A); Raw264.7 insulin-treated (B); PC12 insulin-treated (C); NIH3T3 UV-treated (D) whole cell lysates.

Immunohistochemical analysis of AS160 (pT642) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of AS160 (pT642) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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TBC1D4 / AS160 Antibody (Internal) LS-C368534
LifeSpan Biosciences
catalog: LS-C368534
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of AS160 expression in HeLa (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of AS160 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of AS160 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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TBC1D4 / AS160 Antibody (580-660 aa) LS-C381713
LifeSpan Biosciences
catalog: LS-C381713
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-TBC1D4 (T642) antibody
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TBC1D4 / AS160 Antibody (580-660 aa) LS-C386315
LifeSpan Biosciences
catalog: LS-C386315
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry
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