MELK Antibody (aa639-651) LS-C20179
LifeSpan Biosciences
catalog: LS-C20179
domestic goat polyclonal
reactivity: human
application: western blot

(0.1 ug/ml) Staining of Human Testis lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.
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MELK Antibody (aa201-230) LS-C98360
LifeSpan Biosciences
catalog: LS-C98360
domestic rabbit polyclonal
reactivity: human
application: western blot

The anti-MRLK antibody is used in Western blot to detect MRLK in mouse brain tissue lysate
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MELK Antibody (aa525-554) LS-C98361
LifeSpan Biosciences
catalog: LS-C98361
domestic rabbit polyclonal
reactivity: human
application: western blot

The anti-MELK antibody is used in Western blot to detect MKEL in HEPG2 tissue lysate
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MELK Antibody (aa432-481) LS-C111095
LifeSpan Biosciences
catalog: LS-C111095
domestic rabbit polyclonal
reactivity: human
application: western blot
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MELK Antibody (aa431-480) LS-C119044
LifeSpan Biosciences
catalog: LS-C119044
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from K562 cells, using MELK Antibody. The lane on the right is blocked with the synthesized peptide.
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MELK Antibody (clone 2G2) LS-C169088
LifeSpan Biosciences
catalog: LS-C169088
mouse monoclonal (2G2)
reactivity: human
application: ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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MELK Antibody (Asn449) LS-C176932
LifeSpan Biosciences
catalog: LS-C176932
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

IHC of MELK (N449) pAb in paraffin-embedded human liver carcinoma tissue.
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MELK Antibody (aa21-70) LS-C201506
LifeSpan Biosciences
catalog: LS-C201506
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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MELK Antibody (aa201-230, AP) LS-C279437
LifeSpan Biosciences
catalog: LS-C279437
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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MELK Antibody (aa201-230, APC) LS-C279438
LifeSpan Biosciences
catalog: LS-C279438
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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MELK Antibody (aa201-230, Biotin) LS-C279439
LifeSpan Biosciences
catalog: LS-C279439
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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MELK Antibody (aa525-554, AP) LS-C279440
LifeSpan Biosciences
catalog: LS-C279440
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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MELK Antibody (aa525-554, APC) LS-C279441
LifeSpan Biosciences
catalog: LS-C279441
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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MELK Antibody (aa525-554, Biotin) LS-C279442
LifeSpan Biosciences
catalog: LS-C279442
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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MELK Antibody (aa525-554, FITC) LS-C279443
LifeSpan Biosciences
catalog: LS-C279443
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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MELK Antibody (aa525-554, Azide-free, HRP) LS-C279444
LifeSpan Biosciences
catalog: LS-C279444
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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MELK Antibody (aa525-554, PE) LS-C279445
LifeSpan Biosciences
catalog: LS-C279445
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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MELK Antibody (aa201-230, FITC) LS-C279446
LifeSpan Biosciences
catalog: LS-C279446
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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MELK Antibody (aa201-230, Azide-free, HRP) LS-C279447
LifeSpan Biosciences
catalog: LS-C279447
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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MELK Antibody (aa201-230, PE) LS-C279448
LifeSpan Biosciences
catalog: LS-C279448
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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MELK Antibody (aa425-475) LS-C289836
LifeSpan Biosciences
catalog: LS-C289836
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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MELK Antibody (aa550-650) LS-C309209
LifeSpan Biosciences
catalog: LS-C309209
mouse monoclonal
reactivity: human
application: ELISA
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MELK Antibody (aa201-230) LS-C327886
LifeSpan Biosciences
catalog: LS-C327886
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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MELK Antibody (aa525-554) LS-C327888
LifeSpan Biosciences
catalog: LS-C327888
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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MELK Antibody (Internal) LS-C345685
LifeSpan Biosciences
catalog: LS-C345685
domestic rabbit polyclonal
reactivity: human
application: western blot
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MELK Antibody (Internal) LS-C354135
LifeSpan Biosciences
catalog: LS-C354135
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of MELK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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MELK Antibody (aa434-468) LS-C356499
LifeSpan Biosciences
catalog: LS-C356499
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Anti-Melk Antibody at 1:1000 dilution + mouse ovary lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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MELK Antibody (aa381-415) LS-C356569
LifeSpan Biosciences
catalog: LS-C356569
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

All lanes : Anti-Melk Antibody at 1:1000 dilution Lane 1: mouse spleen lysates Lane 2: mouse thymus lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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MELK Antibody (400-480 aa, Internal) LS-C384511
LifeSpan Biosciences
catalog: LS-C384511
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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