HDAC9 Antibody (aa1017-1066) LS-C118483
LifeSpan Biosciences
catalog: LS-C118483
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HepG2 cells, using HDAC9 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HDAC9 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 cells, using HDAC9 Antibody. The lane on the right is blocked with the synthesized peptide.
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HDAC9 Antibody (C-Terminus) LS-C40398
LifeSpan Biosciences
catalog: LS-C40398
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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HDAC9 Antibody (C-Terminus) LS-C110721
LifeSpan Biosciences
catalog: LS-C110721
domestic rabbit polyclonal
reactivity: human
application: western blot
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HDAC9 Antibody (aa2-32) LS-C96661
LifeSpan Biosciences
catalog: LS-C96661
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Figure 1: Immunoblots for MITR / HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.

This figure shows that both antibody can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 transfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).

Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a FLAG antibody (bottom panel) detecting a FLAG epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
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HDAC9 Antibody (aa503-533) LS-C96662
LifeSpan Biosciences
catalog: LS-C96662
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Both anti-HDAC9 N-term (AP1109a) and C-term antibody were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both antibody specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom figure shows that both antibody can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 transfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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HDAC9 Antibody LS-C48566
LifeSpan Biosciences
catalog: LS-C48566
domestic rabbit polyclonal
reactivity: human
application: western blot
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HDAC9 Antibody (aa100-150) LS-C148570
LifeSpan Biosciences
catalog: LS-C148570
domestic rabbit polyclonal
reactivity: human
application: western blot
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HDAC9 Antibody (Pro1047) LS-C176063
LifeSpan Biosciences
catalog: LS-C176063
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of HDAC9 (P1047) pAb in extracts from HepG2 cells.

IHC of HDAC9 (P1047) pAb in paraffin-embedded human lung carcinoma tissue.
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HDAC9 Antibody (aa503-533, AP) LS-C236144
LifeSpan Biosciences
catalog: LS-C236144
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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HDAC9 Antibody (aa503-533, APC) LS-C236145
LifeSpan Biosciences
catalog: LS-C236145
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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HDAC9 Antibody (aa503-533, Biotin) LS-C236146
LifeSpan Biosciences
catalog: LS-C236146
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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HDAC9 Antibody (aa503-533, FITC) LS-C236148
LifeSpan Biosciences
catalog: LS-C236148
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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HDAC9 Antibody (aa503-533, PE) LS-C236149
LifeSpan Biosciences
catalog: LS-C236149
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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HDAC9 Antibody (aa2-32, AP) LS-C236150
LifeSpan Biosciences
catalog: LS-C236150
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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HDAC9 Antibody (aa2-32, APC) LS-C236151
LifeSpan Biosciences
catalog: LS-C236151
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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HDAC9 Antibody (aa2-32, Biotin) LS-C236152
LifeSpan Biosciences
catalog: LS-C236152
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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HDAC9 Antibody (aa2-32, FITC) LS-C236153
LifeSpan Biosciences
catalog: LS-C236153
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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HDAC9 Antibody (aa2-32, PE) LS-C236157
LifeSpan Biosciences
catalog: LS-C236157
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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HDAC9 Antibody (aa503-533, Azide-free, HRP) LS-C249170
LifeSpan Biosciences
catalog: LS-C249170
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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HDAC9 Antibody (aa2-32, Azide-free, HRP) LS-C249174
LifeSpan Biosciences
catalog: LS-C249174
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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IHC-plus™ HDAC9 Antibody (N-Terminus) LS-B10810
LifeSpan Biosciences
catalog: LS-B10810
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-HDAC9 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ HDAC9 Antibody (Internal) LS-B10811
LifeSpan Biosciences
catalog: LS-B10811
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-HDAC9 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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HDAC9 Antibody LS-C308198
LifeSpan Biosciences
catalog: LS-C308198
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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HDAC9 Antibody (aa481-570) LS-C308204
LifeSpan Biosciences
catalog: LS-C308204
mouse monoclonal
reactivity: human
application: ELISA, immunocytochemistry
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HDAC9 Antibody LS-C193000
LifeSpan Biosciences
catalog: LS-C193000
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot.
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HDAC9 Antibody (aa503-533) LS-C321909
LifeSpan Biosciences
catalog: LS-C321909
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation
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HDAC9 Antibody (aa2-32) LS-C321911
LifeSpan Biosciences
catalog: LS-C321911
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
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HDAC9 Antibody LS-C331507
LifeSpan Biosciences
catalog: LS-C331507
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cell lines, using HDAC9 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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HDAC9 Antibody (aa181-460, clone OTI6E2) LS-C339514
LifeSpan Biosciences
catalog: LS-C339514
mouse monoclonal (OTI6E2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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HDAC9 Antibody (aa181-460, clone OTI6G4) LS-C339542
LifeSpan Biosciences
catalog: LS-C339542
mouse monoclonal (OTI6G4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.
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HDAC9 Antibody (aa181-460, clone OTI3D10) LS-C339543
LifeSpan Biosciences
catalog: LS-C339543
mouse monoclonal (OTI3D10)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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HDAC9 Antibody (aa181-460, clone OTI7A5) LS-C339551
LifeSpan Biosciences
catalog: LS-C339551
mouse monoclonal (OTI7A5)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.
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HDAC9 Antibody (aa181-460, clone OTI5B8) LS-C339557
LifeSpan Biosciences
catalog: LS-C339557
mouse monoclonal (OTI5B8)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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HDAC9 Antibody (aa181-460, clone OTI7G2) LS-C339558
LifeSpan Biosciences
catalog: LS-C339558
mouse monoclonal (OTI7G2)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9.

IHC of paraffin-embedded Human breast tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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HDAC9 Antibody (C-Terminus) LS-C353208
LifeSpan Biosciences
catalog: LS-C353208
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Histone Deacetylase 9 expression in LO2 (A); mouse heart (B); rat heart (C); Ramos (D) whole cell lysates.

Immunohistochemical analysis of Histone Deacetylase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone Deacetylase 9 staining in Ramos cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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HDAC9 Antibody LS-C356801
LifeSpan Biosciences
catalog: LS-C356801
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysates from HeLa,K562 cell line (from left to right), using HDAC9 Antibody(A1-0639). A1-0639 was diluted at 1:500 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20 ug per lane.
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Histone Deacetylase HDAC5 / HDAC9 Antibody (phospho-Ser259/Ser220) LS-C358772
LifeSpan Biosciences
catalog: LS-C358772
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of Histone Deacetylase 5/9 (pS259/220) expression in PC3 (A); HeLa (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of Histone Deacetylase 5/9 (pS259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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HDAC9 Antibody (aa23-343) LS-C373313
LifeSpan Biosciences
catalog: LS-C373313
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of HDAC9 antibody.

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HDAC9 Antibody (980-1060 aa, C-terminal) LS-C383931
LifeSpan Biosciences
catalog: LS-C383931
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Histone deacetylase 9 antibody
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