IHC-plus™ ESPL1 / Separase Antibody (clone 6H6) LS-B6199
LifeSpan Biosciences
catalog: LS-B6199
mouse monoclonal (6H6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of ESPL1 expression in HeLa NE.

Anti-ESPL1 / Separase antibody IHC of human tonsil, mitotic figures. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
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ESPL1 / Separase Antibody (aa1866-1996, clone XJ11-4D7) LS-C2889
LifeSpan Biosciences
catalog: LS-C2889
mouse monoclonal (XJ11-4D7)
reactivity: human
application: western blot, immunocytochemistry
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ESPL1 / Separase Antibody (aa1866-1996, clone XJ11-1B12) LS-C2890
LifeSpan Biosciences
catalog: LS-C2890
mouse monoclonal (XJ11-1B12)
reactivity: human
application: western blot, immunocytochemistry
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ESPL1 / Separase Antibody (Internal) LS-C91293
LifeSpan Biosciences
catalog: LS-C91293
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
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ESPL1 / Separase Antibody (C-Terminus) LS-C91294
LifeSpan Biosciences
catalog: LS-C91294
mouse monoclonal
reactivity: human
application: immunocytochemistry
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ESPL1 / Separase Antibody (C-Terminus) LS-C91295
LifeSpan Biosciences
catalog: LS-C91295
mouse monoclonal
reactivity: human
application: western blot
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ESPL1 / Separase Antibody (phospho-Ser1126) LS-C96968
LifeSpan Biosciences
catalog: LS-C96968
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Dot blot of anti-hSeparase-S801 Phospho-specific antibody on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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ESPL1 / Separase Antibody LS-C126268
LifeSpan Biosciences
catalog: LS-C126268
mouse monoclonal
reactivity: human
application: western blot
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ESPL1 / Separase Antibody (C-Terminus, clone XJ11-1B12, Biotin) LS-C141149
LifeSpan Biosciences
catalog: LS-C141149
mouse monoclonal (XJ11-1B12)
reactivity: human
conjugate: biotin
application: western blot, immunocytochemistry
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ESPL1 / Separase Antibody (C-Terminus, clone XJ11-1B12, DY650) LS-C141150
LifeSpan Biosciences
catalog: LS-C141150
mouse monoclonal (XJ11-1B12)
reactivity: human
application: western blot, immunocytochemistry
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ESPL1 / Separase Antibody (C-Terminus, clone XJ11-1B12, DY488) LS-C141151
LifeSpan Biosciences
catalog: LS-C141151
mouse monoclonal (XJ11-1B12)
reactivity: human
application: western blot, immunocytochemistry
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ESPL1 / Separase Antibody (C-Terminus, clone XJ11-1B12, DY550) LS-C141153
LifeSpan Biosciences
catalog: LS-C141153
mouse monoclonal (XJ11-1B12)
reactivity: human
application: western blot, immunocytochemistry
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ESPL1 / Separase Antibody (Internal) LS-C141881
LifeSpan Biosciences
catalog: LS-C141881
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ ESPL1 / Separase Antibody (aa767-816) LS-B8366
LifeSpan Biosciences
catalog: LS-B8366
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-Separase antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

Immunofluorescence analysis of HUVEC cells, using SEPARASE Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using SEPARASE Antibody. The lane on the right is blocked with the synthesized peptide.
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ESPL1 / Separase Antibody (Ala795) LS-C175888
LifeSpan Biosciences
catalog: LS-C175888
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of Separase (A795) pAb in extracts from HEK293A and Raw264.7 cells.
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ESPL1 / Separase Antibody (Ala795) LS-C176573
LifeSpan Biosciences
catalog: LS-C176573
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Separase (A795) pAb in extracts from 293 cells.

IHC of Separase (A795) pAb in paraffin-embedded human colon carcinoma tissue.
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ESPL1 / Separase Antibody (clone 2329C1a) LS-C184740
LifeSpan Biosciences
catalog: LS-C184740
mouse monoclonal (2329C1a)
reactivity: human
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ESPL1 / Separase Antibody (phospho-Ser801) LS-C199560
LifeSpan Biosciences
catalog: LS-C199560
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.
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ESPL1 / Separase Antibody (phospho-Ser1176, APC) LS-C282598
LifeSpan Biosciences
catalog: LS-C282598
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry
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ESPL1 / Separase Antibody (phospho-Ser1176, AP) LS-C282599
LifeSpan Biosciences
catalog: LS-C282599
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry
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ESPL1 / Separase Antibody (phospho-Ser1176, FITC) LS-C282600
LifeSpan Biosciences
catalog: LS-C282600
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry
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ESPL1 / Separase Antibody (phospho-Ser1176, Azide-free, HRP) LS-C282601
LifeSpan Biosciences
catalog: LS-C282601
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1176, PE) LS-C282602
LifeSpan Biosciences
catalog: LS-C282602
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry
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ESPL1 / Separase Antibody (phospho-Ser748, AP) LS-C282603
LifeSpan Biosciences
catalog: LS-C282603
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser748, APC) LS-C282604
LifeSpan Biosciences
catalog: LS-C282604
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser748, Biotin) LS-C282605
LifeSpan Biosciences
catalog: LS-C282605
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser748, Azide-free, HRP) LS-C282606
LifeSpan Biosciences
catalog: LS-C282606
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry
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ESPL1 / Separase Antibody (phospho-Ser748, FITC) LS-C282607
LifeSpan Biosciences
catalog: LS-C282607
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser748, PE) LS-C282608
LifeSpan Biosciences
catalog: LS-C282608
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser801, AP) LS-C282609
LifeSpan Biosciences
catalog: LS-C282609
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser801, APC) LS-C282610
LifeSpan Biosciences
catalog: LS-C282610
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry
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ESPL1 / Separase Antibody (phospho-Ser801, Biotin) LS-C282611
LifeSpan Biosciences
catalog: LS-C282611
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser801, FITC) LS-C282612
LifeSpan Biosciences
catalog: LS-C282612
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser801, Azide-free, HRP) LS-C282613
LifeSpan Biosciences
catalog: LS-C282613
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser801, PE) LS-C282614
LifeSpan Biosciences
catalog: LS-C282614
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1501, AP) LS-C282615
LifeSpan Biosciences
catalog: LS-C282615
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1501, APC) LS-C282616
LifeSpan Biosciences
catalog: LS-C282616
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1501, Biotin) LS-C282617
LifeSpan Biosciences
catalog: LS-C282617
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1501, FITC) LS-C282618
LifeSpan Biosciences
catalog: LS-C282618
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1501, Azide-free, HRP) LS-C282619
LifeSpan Biosciences
catalog: LS-C282619
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1501, PE) LS-C282620
LifeSpan Biosciences
catalog: LS-C282620
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126, AP) LS-C282621
LifeSpan Biosciences
catalog: LS-C282621
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126, APC) LS-C282622
LifeSpan Biosciences
catalog: LS-C282622
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126, Biotin) LS-C282623
LifeSpan Biosciences
catalog: LS-C282623
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126, Azide-free, HRP) LS-C282624
LifeSpan Biosciences
catalog: LS-C282624
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry
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price:
to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126, FITC) LS-C282626
LifeSpan Biosciences
catalog: LS-C282626
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126, PE) LS-C282627
LifeSpan Biosciences
catalog: LS-C282627
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1176, Biotin) LS-C284695
LifeSpan Biosciences
catalog: LS-C284695
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser748) LS-C328625
LifeSpan Biosciences
catalog: LS-C328625
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser801) LS-C328626
LifeSpan Biosciences
catalog: LS-C328626
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1501) LS-C328628
LifeSpan Biosciences
catalog: LS-C328628
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126) LS-C328630
LifeSpan Biosciences
catalog: LS-C328630
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1176) LS-C330649
LifeSpan Biosciences
catalog: LS-C330649
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry
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to the supplier
ESPL1 / Separase Antibody (phospho-Ser1126) LS-C336094
LifeSpan Biosciences
catalog: LS-C336094
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of U2OS cells.
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ESPL1 / Separase Antibody (phospho-Ser801) LS-C354138
LifeSpan Biosciences
catalog: LS-C354138
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Separase (pS801) expression in MDA-MB-231 (A); A431 (B) whole cell lysates.

Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Separase (pS801) staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ESPL1 / Separase Antibody (Internal) LS-C358749
LifeSpan Biosciences
catalog: LS-C358749
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Separase expression in A431 (A); HUVEC (B) whole cell lysates.

Immunohistochemical analysis of Separase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Separase staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ ESPL1 / Separase Antibody (N-Terminus) LS-B13240
LifeSpan Biosciences
catalog: LS-B13240
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
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ESPL1 / Separase Antibody (740-820 aa) LS-C381459
LifeSpan Biosciences
catalog: LS-C381459
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Phospho-Separase (S801) antibody
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ESPL1 / Separase Antibody (740-820 aa) LS-C386024
LifeSpan Biosciences
catalog: LS-C386024
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Separase antibody
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