LifeSpan Biosciences ATG5 antibody

IHC-plus™ APG5 / ATG5 Antibody (C-Terminus) LS-B4678
LifeSpan Biosciences
catalog: LS-B4678

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATG5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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IHC-plus™ APG5 / ATG5 Antibody LS-B1843
LifeSpan Biosciences
catalog: LS-B1843

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATG5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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APG5 / ATG5 Antibody (C-Terminus) LS-C69125
LifeSpan Biosciences
catalog: LS-C69125

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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APG5 / ATG5 Antibody LS-C156406
LifeSpan Biosciences
catalog: LS-C156406

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of lysate from K562 cell line, using APG5 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

ATG5 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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APG5 / ATG5 Antibody (aa209-238) LS-C156610
LifeSpan Biosciences
catalog: LS-C156610

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Fluorescent image of U251 cells stained with ATG5 Antibody. Antibody was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 mu M, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Cos7, HEK293, MEF, and HeLa cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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APG5 / ATG5 Antibody LS-C53246
LifeSpan Biosciences
catalog: LS-C53246

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ATG5 in rat spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence of ATG5 in Human Spleen cells with ATG5 antibody at 20 ug/ml.

Immunohistochemistry of ATG5 in human spleen tissue with ATG5 antibody at 2.5 µg/mL.
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APG5 / ATG5 Antibody (C-Terminus) LS-C69129
LifeSpan Biosciences
catalog: LS-C69129

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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APG5 / ATG5 Antibody (C-Terminus) LS-C69130
LifeSpan Biosciences
catalog: LS-C69130

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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APG5 / ATG5 Antibody (aa1-50, Biotin) LS-C83781
LifeSpan Biosciences
catalog: LS-C83781

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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APG5 / ATG5 Antibody LS-C108463
LifeSpan Biosciences
catalog: LS-C108463

chicken polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Western blot of ATG5 in mouse spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 2 ug/ml. Below: Immunohistochemistry of ATG5 in human spleen tissue with ATG5 antibody at 2.5 ug/ml.

Immunohistochemistry of ATG5 in human spleen tissue with ATG5 antibody at 2.5 µg/mL.
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APG5 / ATG5 Antibody (aa139-152) LS-C112716
LifeSpan Biosciences
catalog: LS-C112716

domestic goat polyclonal
reactivity: human

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APG5 / ATG5 Antibody (aa161-210) LS-C117039
LifeSpan Biosciences
catalog: LS-C117039

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG5 / ATG5 Antibody (aa1-50, HRP) LS-C122681
LifeSpan Biosciences
catalog: LS-C122681

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, immunohistochemistry

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APG5 / ATG5 Antibody (aa1-50, Biotin) LS-C122682
LifeSpan Biosciences
catalog: LS-C122682

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, immunohistochemistry

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APG5 / ATG5 Antibody LS-C125093
LifeSpan Biosciences
catalog: LS-C125093

mouse monoclonal
reactivity: human
application: western blot, ELISA

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APG5 / ATG5 Antibody LS-C125094
LifeSpan Biosciences
catalog: LS-C125094

mouse monoclonal
reactivity: human
application: western blot, ELISA

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APG5 / ATG5 Antibody (N-Terminus, DY650) LS-C141450
LifeSpan Biosciences
catalog: LS-C141450

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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APG5 / ATG5 Antibody (N-Terminus, DY488) LS-C141451
LifeSpan Biosciences
catalog: LS-C141451

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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APG5 / ATG5 Antibody (N-Terminus, DY550) LS-C141452
LifeSpan Biosciences
catalog: LS-C141452

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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APG5 / ATG5 Antibody (clone 1G11) LS-C139266
LifeSpan Biosciences
catalog: LS-C139266

mouse monoclonal (1G11)
reactivity: human
application: western blot, ELISA

ATG5 monoclonal antibody (M04), clone 1G11. Western blot of ATG5 expression in Raw 264.7.
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APG5 / ATG5 Antibody (clone 3E1) LS-C139274
LifeSpan Biosciences
catalog: LS-C139274

mouse monoclonal (3E1)
reactivity: human
application: western blot, ELISA

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APG5 / ATG5 Antibody (clone 2G8) LS-C139288
LifeSpan Biosciences
catalog: LS-C139288

mouse monoclonal (2G8)
reactivity: human
application: western blot, ELISA

ATG5 monoclonal antibody (M11), clone 2G8. Western blot of ATG5 expression in Jurkat.
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APG5 / ATG5 Antibody (clone 3G11) LS-C139307
LifeSpan Biosciences
catalog: LS-C139307

mouse monoclonal (3G11)
reactivity: human
application: western blot, ELISA

ATG5 monoclonal antibody (M06), clone 3G11. Western blot of ATG5 expression in Raw 264.7.
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APG5 / ATG5 Antibody (clone 4B7) LS-C139446
LifeSpan Biosciences
catalog: LS-C139446

mouse monoclonal (4B7)
reactivity: human
application: western blot, ELISA

ATG5 monoclonal antibody (M10), clone 4B7. Western blot of ATG5 expression in Jurkat.
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APG5 / ATG5 Antibody (clone 4E7) LS-C139447
LifeSpan Biosciences
catalog: LS-C139447

mouse monoclonal (4E7)
reactivity: human
application: western blot, ELISA

ATG5 monoclonal antibody (M09), clone 4E7. Western blot of ATG5 expression in Jurkat.
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APG5 / ATG5 Antibody (clone 1E7) LS-C139448
LifeSpan Biosciences
catalog: LS-C139448

mouse monoclonal (1E7)
reactivity: human
application: western blot, ELISA

ATG5 monoclonal antibody (M05), clone 1E7. Western blot of ATG5 expression in Raw 264.7.
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APG5 / ATG5 Antibody (clone 1C4) LS-C139449
LifeSpan Biosciences
catalog: LS-C139449

mouse monoclonal (1C4)
reactivity: human
application: western blot, ELISA

Western blot of ATG5 expression in transfected 293T cell line by ATG5 monoclonal antibody (M02), clone 1C4.
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IHC-plus™ APG5 / ATG5 Antibody (aa8-318) LS-B7518
LifeSpan Biosciences
catalog: LS-B7518

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-ATG5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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APG5 / ATG5 Antibody (clone 22CT1.11.4.4, AP) LS-C212418
LifeSpan Biosciences
catalog: LS-C212418

mouse monoclonal (22CT1.11.4.4)
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (clone 22CT1.11.4.4, Biotin) LS-C212419
LifeSpan Biosciences
catalog: LS-C212419

mouse monoclonal (22CT1.11.4.4)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (clone 22CT1.11.4.4, Azide-free, HRP) LS-C212420
LifeSpan Biosciences
catalog: LS-C212420

mouse monoclonal (22CT1.11.4.4)
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (clone 22CT1.11.4.4, APC) LS-C212421
LifeSpan Biosciences
catalog: LS-C212421

mouse monoclonal (22CT1.11.4.4)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (clone 22CT1.11.4.4, FITC) LS-C212422
LifeSpan Biosciences
catalog: LS-C212422

mouse monoclonal (22CT1.11.4.4)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (clone 22CT1.11.4.4, PE) LS-C212423
LifeSpan Biosciences
catalog: LS-C212423

mouse monoclonal (22CT1.11.4.4)
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (AP) LS-C212745
LifeSpan Biosciences
catalog: LS-C212745

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (APC) LS-C212746
LifeSpan Biosciences
catalog: LS-C212746

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (FITC) LS-C212747
LifeSpan Biosciences
catalog: LS-C212747

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (PE) LS-C212748
LifeSpan Biosciences
catalog: LS-C212748

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (Tyr35, AP) LS-C212753
LifeSpan Biosciences
catalog: LS-C212753

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (Tyr35, APC) LS-C212754
LifeSpan Biosciences
catalog: LS-C212754

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (Tyr35, FITC) LS-C212757
LifeSpan Biosciences
catalog: LS-C212757

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (Tyr35, PE) LS-C212758
LifeSpan Biosciences
catalog: LS-C212758

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (aa82-97) LS-C313356
LifeSpan Biosciences
catalog: LS-C313356

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of APG5 / ATG5 antibody. All lanes: Anti- ATG5 at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 4oug. Lane 2: Rat Spleen Tissue Lysate at 4oug. Lane 3: Rat Kidney Tissue Lysate at 4oug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: RAJI Whole Cell Lysate at 40ug. Lane 6: NIH Whole Cell Lysate at 40ug. Lane 7: HEPG2 Whole Cell Lysate at 40ug. Lane 8: PC12 Whole Cell Lysate at 40ug. Lane 9: NRK Whole Cell Lysate at 40ug. Predicted bind size: 32KD. Observed bind size: 32KD.
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APG5 / ATG5 Antibody (clone 22CT1.11.4.4) LS-C319112
LifeSpan Biosciences
catalog: LS-C319112

mouse monoclonal (22CT1.11.4.4)
reactivity: human
application: western blot, ELISA, immunohistochemistry

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APG5 / ATG5 Antibody (Tyr35) LS-C319531
LifeSpan Biosciences
catalog: LS-C319531

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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APG5 / ATG5 Antibody LS-C319534
LifeSpan Biosciences
catalog: LS-C319534

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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IHC-plus™ APG5 / ATG5 Antibody (aa1-30) LS-B11793
LifeSpan Biosciences
catalog: LS-B11793

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot of APG5L Antibody (D3) (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.

APG5 / ATG5 Antibody (aa128-160) LS-C356456
LifeSpan Biosciences
catalog: LS-C356456

domestic rabbit polyclonal
reactivity: human, zebrafish
application: western blot

Anti- (DANRE) atg5 Antibody at 1:1000 dilution + HT-1080 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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APG5 / ATG5 Antibody (aa167-199) LS-C356682
LifeSpan Biosciences
catalog: LS-C356682

domestic rabbit polyclonal
reactivity: human, zebrafish
application: western blot

Anti-atg5 Antibody at 1:4000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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IHC-plus™ APG5 / ATG5 Antibody (aa1-50) LS-B12860
LifeSpan Biosciences
catalog: LS-B12860

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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