IHC-plus™ KLF4 Antibody (aa1-170, clone AT4E6) LS-B2966
LifeSpan Biosciences
catalog: LS-B2966
mouse monoclonal (AT4E6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-KLF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Cell lysates of HeLa and A549 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KLF4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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IHC-plus™ KLF4 Antibody (aa80-129) LS-B1192
LifeSpan Biosciences
catalog: LS-B1192
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-KLF4 antibody IHC of human melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ KLF4 Antibody (aa401-450) LS-B5641
LifeSpan Biosciences
catalog: LS-B5641
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-KLF4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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KLF4 Antibody (aa28-38) LS-C8370
LifeSpan Biosciences
catalog: LS-C8370
domestic rabbit polyclonal
reactivity: human
application: ELISA
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KLF4 Antibody (aa11-22) LS-C55037
LifeSpan Biosciences
catalog: LS-C55037
domestic goat polyclonal
reactivity: human
application: western blot

Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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KLF4 Antibody (clone 56CT5.1.6) LS-C96469
LifeSpan Biosciences
catalog: LS-C96469
mouse monoclonal (56CT5.1.6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
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KLF4 Antibody (aa69-101) LS-C98588
LifeSpan Biosciences
catalog: LS-C98588
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of KLF4 Antibody (N-term C74) in Jurkat,293 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.

Fluorescent confocal image of HeLa cells stained KLF4 (N-term C74) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized to the nuclei in HeLa cells.

Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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KLF4 Antibody LS-C98590
LifeSpan Biosciences
catalog: LS-C98590
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of lysate from HT29 cell line, using KLF4 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Fluorescent confocal image of HeLa cells stained KLF4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized specifically to the nuclei in HeLa cells.

Formalin-fixed and paraffin-embedded human colon carcinoma with KLF4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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KLF4 Antibody (Ser457) LS-C98591
LifeSpan Biosciences
catalog: LS-C98591
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Fluorescent confocal image of HeLa cells stained KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.

Western blot of KLF4-S457 in MDA-MB231, NCI-H460 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.
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KLF4 Antibody LS-C108660
LifeSpan Biosciences
catalog: LS-C108660
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Immunohistochemistry of KLF4 in rat liver tissue with KLF4 antibody at 5 ug/ml.

Western blot analysis of KLF4 in human liver tissue lysate with KLF4 antibody at (A) 1 and (B) 2 ug/ml.
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KLF4 Antibody (aa251-300) LS-C116050
LifeSpan Biosciences
catalog: LS-C116050
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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KLF4 Antibody (N-Terminus) LS-C135057
LifeSpan Biosciences
catalog: LS-C135057
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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KLF4 Antibody LS-C136894
LifeSpan Biosciences
catalog: LS-C136894
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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KLF4 Antibody (clone 4G6E11) LS-C144495
LifeSpan Biosciences
catalog: LS-C144495
mouse monoclonal (4G6E11)
reactivity: human
application: western blot, ELISA

Western blot analysis of KLF4 in mouse liver tissue lysate with KLF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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KLF4 Antibody (clone 4E5C3) LS-C144496
LifeSpan Biosciences
catalog: LS-C144496
mouse monoclonal (4E5C3)
reactivity: human
application: western blot, ELISA

Western blot analysis of KLF4 in mouse liver tissue lysate with KLF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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KLF4 Antibody LS-C146849
LifeSpan Biosciences
catalog: LS-C146849
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
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KLF4 Antibody (aa394-421) LS-C165557
LifeSpan Biosciences
catalog: LS-C165557
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

KLF4 Antibody western blot of A431,MCF-7 cell line lysates (35 ug/lane). The KLF4 antibody detected the KLF4 protein (arrow).

Fluorescent confocal image of SY5Y cells stained KLF4 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

KLF4 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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KLF4 Antibody (clone 1E5) LS-C169232
LifeSpan Biosciences
catalog: LS-C169232
mouse monoclonal (1E5)
reactivity: human
application: western blot, ELISA, immunohistochemistry
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KLF4 Antibody (clone 1E6) LS-C169255
LifeSpan Biosciences
catalog: LS-C169255
mouse monoclonal (1E6)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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KLF4 Antibody (aa283-314) LS-C203673
LifeSpan Biosciences
catalog: LS-C203673
domestic rabbit polyclonal
reactivity: human
application: western blot

KLF4 Antibody western blot of MCF-7,K562 cell line lysates (35 ug/lane). The KLF4 antibody detected the KLF4 protein (arrow).
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KLF4 Antibody (clone 56CT5.1.6, AP) LS-C250279
LifeSpan Biosciences
catalog: LS-C250279
mouse monoclonal (56CT5.1.6)
reactivity: human
conjugate: AP
application: western blot, ELISA, immunocytochemistry, flow cytometry
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KLF4 Antibody (clone 56CT5.1.6, APC) LS-C250280
LifeSpan Biosciences
catalog: LS-C250280
mouse monoclonal (56CT5.1.6)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunocytochemistry, flow cytometry
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KLF4 Antibody (clone 56CT5.1.6, Biotin) LS-C250281
LifeSpan Biosciences
catalog: LS-C250281
mouse monoclonal (56CT5.1.6)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunocytochemistry, flow cytometry
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KLF4 Antibody (clone 56CT5.1.6, FITC) LS-C250282
LifeSpan Biosciences
catalog: LS-C250282
mouse monoclonal (56CT5.1.6)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunocytochemistry, flow cytometry
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KLF4 Antibody (clone 56CT5.1.6, PE) LS-C250285
LifeSpan Biosciences
catalog: LS-C250285
mouse monoclonal (56CT5.1.6)
reactivity: human
conjugate: PE
application: western blot, ELISA, immunocytochemistry, flow cytometry
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KLF4 Antibody (clone 56CT5.1.6, Azide-free, HRP) LS-C250286
LifeSpan Biosciences
catalog: LS-C250286
mouse monoclonal (56CT5.1.6)
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunocytochemistry, flow cytometry
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KLF4 Antibody (Ser457, APC) LS-C261419
LifeSpan Biosciences
catalog: LS-C261419
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunocytochemistry
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KLF4 Antibody (Ser457, AP) LS-C261420
LifeSpan Biosciences
catalog: LS-C261420
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunocytochemistry
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KLF4 Antibody (Ser457, FITC) LS-C261421
LifeSpan Biosciences
catalog: LS-C261421
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunocytochemistry
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KLF4 Antibody (Ser457, Biotin) LS-C261422
LifeSpan Biosciences
catalog: LS-C261422
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunocytochemistry
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KLF4 Antibody (Ser457, Azide-free, HRP) LS-C261423
LifeSpan Biosciences
catalog: LS-C261423
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunocytochemistry
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KLF4 Antibody (Ser457, PE) LS-C261424
LifeSpan Biosciences
catalog: LS-C261424
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunocytochemistry
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KLF4 Antibody (phospho-Ser245, AP) LS-C261425
LifeSpan Biosciences
catalog: LS-C261425
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunocytochemistry
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KLF4 Antibody (phospho-Ser245, APC) LS-C261426
LifeSpan Biosciences
catalog: LS-C261426
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunocytochemistry
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KLF4 Antibody (phospho-Ser245, FITC) LS-C261427
LifeSpan Biosciences
catalog: LS-C261427
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunocytochemistry
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KLF4 Antibody (phospho-Ser245, Biotin) LS-C261428
LifeSpan Biosciences
catalog: LS-C261428
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunocytochemistry
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KLF4 Antibody (phospho-Ser245, Azide-free, HRP) LS-C261429
LifeSpan Biosciences
catalog: LS-C261429
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunocytochemistry
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KLF4 Antibody (phospho-Ser245, PE) LS-C261430
LifeSpan Biosciences
catalog: LS-C261430
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunocytochemistry
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KLF4 Antibody (aa93-108) LS-C313313
LifeSpan Biosciences
catalog: LS-C313313
domestic rabbit polyclonal
reactivity: human
application: western blot

WB of KLF4 antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: U20S Cell Lysate.

KLF4 antibody. IHC(P): Human Tonsil Tissue.

KLF4 antibody. ICC: A549 Cell.
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KLF4 Antibody (clone 56CT5.1.6) LS-C319168
LifeSpan Biosciences
catalog: LS-C319168
mouse monoclonal (56CT5.1.6)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry
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KLF4 Antibody (phospho-Ser245) LS-C322512
LifeSpan Biosciences
catalog: LS-C322512
domestic rabbit polyclonal
reactivity: human
application: ELISA, immunocytochemistry
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KLF4 Antibody (Ser457) LS-C322514
LifeSpan Biosciences
catalog: LS-C322514
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry
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KLF4 Antibody (aa1-50) LS-C47390
LifeSpan Biosciences
catalog: LS-C47390
domestic rabbit polyclonal
reactivity: human
application: western blot
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KLF4 Antibody (aa408-450) LS-C356334
LifeSpan Biosciences
catalog: LS-C356334
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysate from NCCIT cell line, using KLF4 antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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KLF4 Antibody (aa321-354) LS-C356421
LifeSpan Biosciences
catalog: LS-C356421
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of lysate from mouse heart tissue lysate, using Klf4 antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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KLF4 Antibody (N-Terminus) LS-C357947
LifeSpan Biosciences
catalog: LS-C357947
domestic rabbit polyclonal
reactivity: human
application: western blot
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KLF4 Antibody (aa1-300) LS-C358155
LifeSpan Biosciences
catalog: LS-C358155
domestic rabbit polyclonal
reactivity: human
application: western blot
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KLF4 Antibody (aa30-230) LS-C376030
LifeSpan Biosciences
catalog: LS-C376030
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: Krueppel-like factor 4 antibody at 5 ug/ml+293T whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 55 kDa. Observed band size: 55 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human rectum using antibody at 1:100 dilution.
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