IHC-plus™ Securin / PTTG1 Antibody (aa148-160) LS-B5119
LifeSpan Biosciences
catalog: LS-B5119

domestic goat polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-PTTG1 / Securin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

Securin / PTTG1 antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Securin / PTTG1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Securin / PTTG1 Antibody (N-Terminus) LS-C9840
LifeSpan Biosciences
catalog: LS-C9840

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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Securin / PTTG1 Antibody (clone DCS-280) LS-C87799
LifeSpan Biosciences
catalog: LS-C87799

mouse monoclonal (DCS-280)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes in germinal center. This image was taken for the unmodified form of this product. Other forms have not been tested.
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Securin / PTTG1 Antibody (aa16-45) LS-C100534
LifeSpan Biosciences
catalog: LS-C100534

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of U251 cells, using PTTG1 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

Western blot of PTTG1 antibody in Ramos cell line lysates (35 ug/lane). PTTG1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Securin / PTTG1 Antibody LS-C74223
LifeSpan Biosciences
catalog: LS-C74223

mouse monoclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

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Securin / PTTG1 Antibody (Azide-free) LS-C74225
LifeSpan Biosciences
catalog: LS-C74225

mouse monoclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

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Securin / PTTG1 Antibody (aa111-160) LS-C118744
LifeSpan Biosciences
catalog: LS-C118744

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Immunofluorescence analysis of HUVEC cells, using PTTG1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2, Jurkat, and 293 cells, using PTTG1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Securin / PTTG1 Antibody (Internal) LS-C145186
LifeSpan Biosciences
catalog: LS-C145186

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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Securin / PTTG1 Antibody (clone 2C3) LS-C139438
LifeSpan Biosciences
catalog: LS-C139438

mouse monoclonal (2C3)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged PTTG1 is approximately 0.03 ng/ml as a capture antibody.
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Securin / PTTG1 Antibody (Internal) LS-C153548
LifeSpan Biosciences
catalog: LS-C153548

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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Securin / PTTG1 Antibody (Leu149) LS-C176719
LifeSpan Biosciences
catalog: LS-C176719

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of PTTG1/2/3 (L149) pAb in extracts from HepG2 cells.

IHC of PTTG1/2/3 (L149) pAb in paraffin-embedded human testis tissue.
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Securin / PTTG1 Antibody (aa146-161) LS-C313339
LifeSpan Biosciences
catalog: LS-C313339

domestic rabbit polyclonal
reactivity: human
application: western blot

WB of Securin / PTTG1 antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: SKOV-3 Cell Lysate. Lane 5: A575 Cell Lysate.
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Securin / PTTG1 Antibody (Internal) LS-C353139
LifeSpan Biosciences
catalog: LS-C353139

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of PTTG1/2/3 expression in HepG2 (A); Jurkat (B); NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of PTTG1/2/3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of PTTG1/2/3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Securin / PTTG1 Antibody LS-C356967
LifeSpan Biosciences
catalog: LS-C356967

domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-PTTG1/2/3 Antibody at 1:1000 dilution Lane 1: Daudi whole cell lysates Lane 2: HeLa whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Securin / PTTG1 Antibody (aa2-199) LS-C374646
LifeSpan Biosciences
catalog: LS-C374646

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western Blot; Sample: Human 293T cell lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse PTTG1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

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