IHC-plus™ BUB3 Antibody LS-B1340
LifeSpan Biosciences
catalog: LS-B1340

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-BUB3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 ug/ml.

Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 ug/ml.
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IHC-plus™ BUB3 Antibody LS-B4858
LifeSpan Biosciences
catalog: LS-B4858

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Sample (30 ug of whole cell lysate). A: H1299. 10% SDS PAGE. BUB3 antibody diluted at 1:1000.

Anti-BUB3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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BUB3 Antibody (N-Terminus) LS-C136992
LifeSpan Biosciences
catalog: LS-C136992

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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BUB3 Antibody (N-Terminus) LS-C112219
LifeSpan Biosciences
catalog: LS-C112219

domestic rabbit polyclonal
reactivity: human
application: western blot

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BUB3 Antibody (aa279-328) LS-C119398
LifeSpan Biosciences
catalog: LS-C119398

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using BUB3 Antibody. The lane on the right is blocked with the synthesized peptide.
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BUB3 Antibody (aa1-328) LS-C125210
LifeSpan Biosciences
catalog: LS-C125210

domestic goat polyclonal
reactivity: human
application: western blot, ELISA

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BUB3 Antibody (His274) LS-C176965
LifeSpan Biosciences
catalog: LS-C176965

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of Bub3 (H274) pAb in extracts from HeLa cells.

IHC of Bub3 (H274) pAb in paraffin-embedded human brain tissue.
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BUB3 Antibody (aa275-325) LS-C286392
LifeSpan Biosciences
catalog: LS-C286392

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

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BUB3 Antibody (aa1-50) LS-C287258
LifeSpan Biosciences
catalog: LS-C287258

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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BUB3 Antibody (aa76-90) LS-C312796
LifeSpan Biosciences
catalog: LS-C312796

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

WB of BUB3 antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4:COLO320 Cell Lysate.

BUB3 antibody. IHC(P): Human Breast Cancer Tissue.

BUB3 antibody. ICC: HELA Cell.
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BUB3 Antibody LS-C334784
LifeSpan Biosciences
catalog: LS-C334784

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded Rat kidney tissue.

Immunofluorescence analysis of U20S cells.
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BUB3 Antibody (C-Terminus) LS-C353144
LifeSpan Biosciences
catalog: LS-C353144

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of BUB3 expression in HeLa (A); HEK293T (B) whole cell lysates.

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of BUB3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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BUB3 Antibody LS-C356834
LifeSpan Biosciences
catalog: LS-C356834

domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-BUB3 Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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BUB3 Antibody (260-340 aa, C-terminal) LS-C382332
LifeSpan Biosciences
catalog: LS-C382332

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Bub3 antibody
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