IHC-plus™ NEK9 Antibody LS-B1638
LifeSpan Biosciences
catalog: LS-B1638
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-NEK9 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-NEK9 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
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NEK9 Antibody (aa846-877) LS-C101171
LifeSpan Biosciences
catalog: LS-C101171
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

The anti-NEK9 antibody is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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NEK9 Antibody (aa755-804) LS-C111210
LifeSpan Biosciences
catalog: LS-C111210
domestic rabbit polyclonal
reactivity: human
application: western blot
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NEK9 Antibody (aa176-225) LS-C118006
LifeSpan Biosciences
catalog: LS-C118006
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from A549 cells, using NEK9 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ NEK9 Antibody (Pro/Ser/Thr Rich Domain) LS-A7968
LifeSpan Biosciences
catalog: LS-A7968
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-NEK9 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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NEK9 Antibody (aa503-530) LS-C164500
LifeSpan Biosciences
catalog: LS-C164500
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Mouse Nek9 Antibody western blot of human placenta tissue lysates (35 ug/lane). The Nek9 antibody detected the Nek9 protein (arrow).
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NEK9 Antibody (clone OTI7G7) LS-C173217
LifeSpan Biosciences
catalog: LS-C173217
mouse monoclonal (OTI7G7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.

Anti-NEK9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK9 antibody, and then analyzed by flow cytometry.
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NEK9 Antibody (Ser206) LS-C176680
LifeSpan Biosciences
catalog: LS-C176680
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of Nek9 (S206) pAb in extracts from A549 cells.
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NEK9 Antibody (phospho-Thr210) LS-C199306
LifeSpan Biosciences
catalog: LS-C199306
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells, using NEK9 (Phospho-Thr210) Antibody. The lane on the right is blocked with the phospho peptide.
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NEK9 Antibody (aa503-530, AP) LS-C215946
LifeSpan Biosciences
catalog: LS-C215946
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA
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NEK9 Antibody (aa503-530, APC) LS-C215947
LifeSpan Biosciences
catalog: LS-C215947
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA
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NEK9 Antibody (aa503-530, Biotin) LS-C215948
LifeSpan Biosciences
catalog: LS-C215948
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
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NEK9 Antibody (aa503-530, FITC) LS-C215949
LifeSpan Biosciences
catalog: LS-C215949
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA
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NEK9 Antibody (aa503-530, Azide-free, HRP) LS-C215950
LifeSpan Biosciences
catalog: LS-C215950
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA
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NEK9 Antibody (aa503-530, PE) LS-C215953
LifeSpan Biosciences
catalog: LS-C215953
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA
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NEK9 Antibody (aa846-877, AP) LS-C220154
LifeSpan Biosciences
catalog: LS-C220154
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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NEK9 Antibody (aa846-877, APC) LS-C220155
LifeSpan Biosciences
catalog: LS-C220155
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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NEK9 Antibody (aa846-877, Biotin) LS-C220156
LifeSpan Biosciences
catalog: LS-C220156
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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NEK9 Antibody (aa846-877, FITC) LS-C220157
LifeSpan Biosciences
catalog: LS-C220157
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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NEK9 Antibody (aa846-877, Azide-free, HRP) LS-C220158
LifeSpan Biosciences
catalog: LS-C220158
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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NEK9 Antibody (aa846-877, PE) LS-C220160
LifeSpan Biosciences
catalog: LS-C220160
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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NEK9 Antibody (aa675-725) LS-C287965
LifeSpan Biosciences
catalog: LS-C287965
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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NEK9 Antibody (aa929-979) LS-C287967
LifeSpan Biosciences
catalog: LS-C287967
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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NEK9 Antibody (aa503-530) LS-C323293
LifeSpan Biosciences
catalog: LS-C323293
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
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NEK9 Antibody (clone OTI1H1) LS-C337452
LifeSpan Biosciences
catalog: LS-C337452
mouse monoclonal (OTI1H1)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9.

Anti-NEK9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9.
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NEK9 Antibody (C-Terminus) LS-C345689
LifeSpan Biosciences
catalog: LS-C345689
domestic rabbit polyclonal
reactivity: human
application: western blot
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NEK9 Antibody (Kinase Domain) LS-C368260
LifeSpan Biosciences
catalog: LS-C368260
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
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NEK9 Antibody (phospho-Thr210) LS-C368439
LifeSpan Biosciences
catalog: LS-C368439
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of NEK9 (pT210) expression in THP1 (A); HepG2 (B); HeLa (C) whole cell lysates.

Immunohistochemical analysis of NEK9 (pT210) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of NEK9 (pT210) staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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NEK9 Antibody (150-230 aa) LS-C381162
LifeSpan Biosciences
catalog: LS-C381162
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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NEK9 Antibody (150-230 aa) LS-C384816
LifeSpan Biosciences
catalog: LS-C384816
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry
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