IHC-plus™ HRS / HGS Antibody (aa451-500) LS-B5664
LifeSpan Biosciences
catalog: LS-B5664
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-HGS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Immunofluorescence analysis of HUVEC cells, using HGS Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 and HUVEC cells, using HGS Antibody. The lane on the right is blocked with the synthesized peptide.
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HRS / HGS Antibody (aa301-350) LS-C117954
LifeSpan Biosciences
catalog: LS-C117954
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells, using HRS Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HRS Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293, COLO, and HUVEC cells, using HRS Antibody. The lane on the right is blocked with the synthesized peptide.
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HRS / HGS Antibody (aa331-344) LS-C54980
LifeSpan Biosciences
catalog: LS-C54980
domestic goat polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Antibody (2.5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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HRS / HGS Antibody (aa256-286) LS-C99590
LifeSpan Biosciences
catalog: LS-C99590
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of hIMOS-1-Q271 in mouse spleen tissue lysates (35 ug/lane). IMOS-1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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HRS / HGS Antibody (clone OTI1C2) LS-C174537
LifeSpan Biosciences
catalog: LS-C174537
mouse monoclonal (OTI1C2)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HGS.
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HRS / HGS Antibody (clone OTI3H7) LS-C174567
LifeSpan Biosciences
catalog: LS-C174567
mouse monoclonal (OTI3H7)
reactivity: human
application: western blot, immunocytochemistry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HGS.

Anti-HGS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HGS.
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HRS / HGS Antibody (Leu330) LS-C176533
LifeSpan Biosciences
catalog: LS-C176533
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of HRS (L330) pAb in extracts from HUVEC cells.

IHC of HRS (L330) pAb in paraffin-embedded human brain tissue.
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HRS / HGS Antibody (phospho-Tyr216) LS-C178065
LifeSpan Biosciences
catalog: LS-C178065
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot of p-Hrs (Y216) pAb in extracts from A549 cells.
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HRS / HGS Antibody (phospho-Tyr334) LS-C199079
LifeSpan Biosciences
catalog: LS-C199079
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence analysis of HeLa cells treated with Forskolin 40nM 15', using HRS (Phospho-Tyr334) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using HRS (Phospho-Tyr334) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using HRS (Phospho-Tyr334) Antibody. The lane on the right is blocked with the phospho peptide.
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HRS / HGS Antibody (aa164-213) LS-C199890
LifeSpan Biosciences
catalog: LS-C199890
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HeLa cells, using Hrs antibody.
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HRS / HGS Antibody (aa256-286, AP) LS-C278247
LifeSpan Biosciences
catalog: LS-C278247
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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HRS / HGS Antibody (aa256-286, APC) LS-C278248
LifeSpan Biosciences
catalog: LS-C278248
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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HRS / HGS Antibody (aa256-286, Biotin) LS-C278249
LifeSpan Biosciences
catalog: LS-C278249
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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HRS / HGS Antibody (aa256-286, PE) LS-C278250
LifeSpan Biosciences
catalog: LS-C278250
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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HRS / HGS Antibody (aa256-286, FITC) LS-C278251
LifeSpan Biosciences
catalog: LS-C278251
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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HRS / HGS Antibody (aa256-286, Azide-free, HRP) LS-C278253
LifeSpan Biosciences
catalog: LS-C278253
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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HRS / HGS Antibody (aa350-400) LS-C287821
LifeSpan Biosciences
catalog: LS-C287821
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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HRS / HGS Antibody (aa727-C-term) LS-C287823
LifeSpan Biosciences
catalog: LS-C287823
domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation
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HRS / HGS Antibody (phospho-Tyr216) LS-C291502
LifeSpan Biosciences
catalog: LS-C291502
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot analysis of lysates from A549 cells, using phospho-Hrs (Phospho-Tyr216) antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Hrs (Phospho-Tyr216) Antibody
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HRS / HGS Antibody (aa513-612) LS-C308232
LifeSpan Biosciences
catalog: LS-C308232
mouse monoclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry
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HRS / HGS Antibody (aa256-286) LS-C327587
LifeSpan Biosciences
catalog: LS-C327587
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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HRS / HGS Antibody (Internal) LS-C354125
LifeSpan Biosciences
catalog: LS-C354125
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of HGS expression in H1299 (A); HeLa (B) whole cell lysates.

Immunohistochemical analysis of HGS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of HGS staining in H1299 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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IHC-plus™ HRS / HGS Antibody (aa461-510) LS-B12780
LifeSpan Biosciences
catalog: LS-B12780
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of HRS pAb at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: H9C2 whole cell lysate.
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HRS / HGS Antibody (phospho-Tyr216) LS-C380725
LifeSpan Biosciences
catalog: LS-C380725
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA

Western blot of Phospho-Hrs (Y216) antibody
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HRS / HGS Antibody (270-350 aa) LS-C381523
LifeSpan Biosciences
catalog: LS-C381523
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
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HRS / HGS Antibody (420-500 aa, Internal) LS-C384010
LifeSpan Biosciences
catalog: LS-C384010
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Hrs antibody
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HRS / HGS Antibody (270-350 aa) LS-C384012
LifeSpan Biosciences
catalog: LS-C384012
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Hrs antibody
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HRS / HGS Antibody (501-550 aa, Internal) LS-C386908
LifeSpan Biosciences
catalog: LS-C386908
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of Hrs antibody
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