IHC-plus™ APG12 / ATG12 Antibody (Internal) LS-B2140
LifeSpan Biosciences
catalog: LS-B2140

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Anti-ATG12 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ APG12 / ATG12 Antibody LS-B3216
LifeSpan Biosciences
catalog: LS-B3216

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Anti-ATG12 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/ml.
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APG12 / ATG12 Antibody (aa1-30) LS-C156569
LifeSpan Biosciences
catalog: LS-C156569

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Fluorescent image of U251 cells stained with ATG12 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG12 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot of anti-APG12L antibody in HeLa cell lysate. APG12L (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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APG12 / ATG12 Antibody (N-Terminus) LS-C69121
LifeSpan Biosciences
catalog: LS-C69121

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (N-Terminus) LS-C69122
LifeSpan Biosciences
catalog: LS-C69122

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (N-Terminus) LS-C69126
LifeSpan Biosciences
catalog: LS-C69126

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation

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APG12 / ATG12 Antibody (N-Terminus) LS-C69127
LifeSpan Biosciences
catalog: LS-C69127

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation

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APG12 / ATG12 Antibody LS-C53240
LifeSpan Biosciences
catalog: LS-C53240

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 ug/ml.

Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/ml.

Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 µg/mL.
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APG12 / ATG12 Antibody LS-C59542
LifeSpan Biosciences
catalog: LS-C59542

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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APG12 / ATG12 Antibody (aa8-38) LS-C98673
LifeSpan Biosciences
catalog: LS-C98673

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of hAPG12L-T23 in MCF-7 cell line lysates (35 ug/lane). APG12L (arrow) was detected using the purified antibody.

Mouse leukemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L antibody detected punctuate staining indicative of nascent autophagic vacuole structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
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APG12 / ATG12 Antibody (C-Terminus) LS-C117261
LifeSpan Biosciences
catalog: LS-C117261

domestic rabbit polyclonal
reactivity: human
application: western blot

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APG12 / ATG12 Antibody LS-C125098
LifeSpan Biosciences
catalog: LS-C125098

mouse monoclonal
reactivity: human
application: western blot, ELISA

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APG12 / ATG12 Antibody LS-C125099
LifeSpan Biosciences
catalog: LS-C125099

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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APG12 / ATG12 Antibody LS-C126828
LifeSpan Biosciences
catalog: LS-C126828

domestic rabbit monoclonal
reactivity: human
application: western blot, immunoprecipitation

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IHC-plus™ APG12 / ATG12 Antibody (aa79-128) LS-B5897
LifeSpan Biosciences
catalog: LS-B5897

domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ATG12 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APG12 / ATG12 Antibody (clone 1F9) LS-C139421
LifeSpan Biosciences
catalog: LS-C139421

mouse monoclonal (1F9)
reactivity: human
application: western blot, ELISA

Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.
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APG12 / ATG12 Antibody LS-C147032
LifeSpan Biosciences
catalog: LS-C147032

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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APG12 / ATG12 Antibody LS-C156567
LifeSpan Biosciences
catalog: LS-C156567

mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of anti-APG12 Monoclonal Antibody by APG12 recombinant protein. APG12(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Atg12 antibody tested in HEK cells. Fixed in PFA permeablilized with 0.2% Saponin. Blocked and antibodies in 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no leupeptin)(Provided by Nicole McKnight & Sharon Tooze).
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APG12 / ATG12 Antibody (N-Terminus) LS-C204269
LifeSpan Biosciences
catalog: LS-C204269

domestic goat polyclonal
reactivity: human
application: western blot

Western blot. Anti-Atg12 antibody at 1:500 dilution. 293HEK cells transfected with GFP-Atg12. Lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.
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APG12 / ATG12 Antibody (clone 43CT73.3.5.5.4, AP) LS-C232186
LifeSpan Biosciences
catalog: LS-C232186

mouse monoclonal (43CT73.3.5.5.4)
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (clone 43CT73.3.5.5.4, Biotin) LS-C232188
LifeSpan Biosciences
catalog: LS-C232188

mouse monoclonal (43CT73.3.5.5.4)
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (clone 43CT73.3.5.5.4, APC) LS-C232189
LifeSpan Biosciences
catalog: LS-C232189

mouse monoclonal (43CT73.3.5.5.4)
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (clone 43CT73.3.5.5.4, FITC) LS-C232190
LifeSpan Biosciences
catalog: LS-C232190

mouse monoclonal (43CT73.3.5.5.4)
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (clone 43CT73.3.5.5.4, PE) LS-C232191
LifeSpan Biosciences
catalog: LS-C232191

mouse monoclonal (43CT73.3.5.5.4)
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (aa26-56, APC) LS-C232933
LifeSpan Biosciences
catalog: LS-C232933

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (aa26-56, Biotin) LS-C232934
LifeSpan Biosciences
catalog: LS-C232934

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (aa26-56, FITC) LS-C232935
LifeSpan Biosciences
catalog: LS-C232935

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (aa26-56, PE) LS-C232936
LifeSpan Biosciences
catalog: LS-C232936

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (Ser26, AP) LS-C232937
LifeSpan Biosciences
catalog: LS-C232937

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (Ser26, APC) LS-C232938
LifeSpan Biosciences
catalog: LS-C232938

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (Ser26, Biotin) LS-C232939
LifeSpan Biosciences
catalog: LS-C232939

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (Ser26, FITC) LS-C232940
LifeSpan Biosciences
catalog: LS-C232940

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (Ser26, PE) LS-C232942
LifeSpan Biosciences
catalog: LS-C232942

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (aa8-38, AP) LS-C243699
LifeSpan Biosciences
catalog: LS-C243699

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunocytochemistry

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APG12 / ATG12 Antibody (aa8-38, APC) LS-C243700
LifeSpan Biosciences
catalog: LS-C243700

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunocytochemistry

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APG12 / ATG12 Antibody (aa8-38, Biotin) LS-C243701
LifeSpan Biosciences
catalog: LS-C243701

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunocytochemistry

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APG12 / ATG12 Antibody (aa8-38, FITC) LS-C243702
LifeSpan Biosciences
catalog: LS-C243702

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunocytochemistry

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APG12 / ATG12 Antibody (aa8-38, PE) LS-C243705
LifeSpan Biosciences
catalog: LS-C243705

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunocytochemistry

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APG12 / ATG12 Antibody (aa26-56, AP) LS-C246952
LifeSpan Biosciences
catalog: LS-C246952

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (aa8-38, Azide-free, HRP) LS-C247443
LifeSpan Biosciences
catalog: LS-C247443

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunocytochemistry

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APG12 / ATG12 Antibody (clone 43CT73.3.5.5.4, Azide-free, HRP) LS-C249330
LifeSpan Biosciences
catalog: LS-C249330

mouse monoclonal (43CT73.3.5.5.4)
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (aa26-56, Azide-free, HRP) LS-C249479
LifeSpan Biosciences
catalog: LS-C249479

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (Ser26, Azide-free, HRP) LS-C249480
LifeSpan Biosciences
catalog: LS-C249480

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody LS-C306426
LifeSpan Biosciences
catalog: LS-C306426

mouse polyclonal
reactivity: human
application: western blot

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APG12 / ATG12 Antibody LS-C306427
LifeSpan Biosciences
catalog: LS-C306427

mouse monoclonal
reactivity: human
application: western blot, ELISA

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APG12 / ATG12 Antibody (clone 43CT73.3.5.5.4) LS-C319114
LifeSpan Biosciences
catalog: LS-C319114

mouse monoclonal (43CT73.3.5.5.4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

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APG12 / ATG12 Antibody (aa26-56) LS-C319658
LifeSpan Biosciences
catalog: LS-C319658

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (Ser26) LS-C319662
LifeSpan Biosciences
catalog: LS-C319662

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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APG12 / ATG12 Antibody (aa8-38) LS-C326860
LifeSpan Biosciences
catalog: LS-C326860

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

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