IHC-plus™ AIFM1 / AIF / PDCD8 Antibody (aa211-260) LS-B1508
LifeSpan Biosciences
catalog: LS-B1508
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ AIFM1 / AIF / PDCD8 Antibody LS-B3765
LifeSpan Biosciences
catalog: LS-B3765
domestic rabbit polyclonal
reactivity: human, rat
modification: acetylated
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Western blot analysis of AIF in K562 with AIF antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of AIF in K562 cells with AIF antibody at 5 ug/ml.
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AIFM1 / AIF / PDCD8 Antibody (aa151-169) LS-C147989
LifeSpan Biosciences
catalog: LS-C147989
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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AIFM1 / AIF / PDCD8 Antibody (aa593-606) LS-C20360
LifeSpan Biosciences
catalog: LS-C20360
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIFM1 / AIF / PDCD8 Antibody (aa81-130) LS-C40540
LifeSpan Biosciences
catalog: LS-C40540
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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AIFM1 / AIF / PDCD8 Antibody (clone DJ130c) LS-C44950
LifeSpan Biosciences
catalog: LS-C44950
mouse monoclonal (DJ130c)
reactivity: human
application: flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
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AIFM1 / AIF / PDCD8 Antibody LS-C48245
LifeSpan Biosciences
catalog: LS-C48245
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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IHC-plus™ AIFM1 / AIF / PDCD8 Antibody LS-B3744
LifeSpan Biosciences
catalog: LS-B3744
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunocytochemistry of AIF in Jurkat cells with AIF antibody at 2 µg/ml.

Anti-AIFM1 / AIF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of AIF in K562 cells with AIF antibody at 20 ug/ml.
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AIFM1 / AIF / PDCD8 Antibody (C-Terminus) LS-C112280
LifeSpan Biosciences
catalog: LS-C112280
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIFM1 / AIF / PDCD8 Antibody (aa51-100) LS-C119533
LifeSpan Biosciences
catalog: LS-C119533
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from HUVEC cells, HepG2 cells, HeLa cells, and Jurkat cells, using AIFM1 Antibody. The lane on the right is blocked with the synthesized peptide.
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AIFM1 / AIF / PDCD8 Antibody (C-Terminus) LS-C121360
LifeSpan Biosciences
catalog: LS-C121360
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
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AIFM1 / AIF / PDCD8 Antibody LS-C136833
LifeSpan Biosciences
catalog: LS-C136833
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody LS-C137395
LifeSpan Biosciences
catalog: LS-C137395
domestic rabbit polyclonal
reactivity: human
application: western blot
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IHC-plus™ AIFM1 / AIF / PDCD8 Antibody LS-B7901
LifeSpan Biosciences
catalog: LS-B7901
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-AIFM1 / AIF antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
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AIFM1 / AIF / PDCD8 Antibody (aa70-98) LS-C156184
LifeSpan Biosciences
catalog: LS-C156184
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Fluorescent image of U251 cells stained with AIFM1 antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated AIFM1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). AIFM1 immunoreactivity is localized to the cytoplasm of U251 cells.

Western blot of AIFM1 Antibody in 293 cell line lysates (35 ug/lane). AIFM1 (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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AIFM1 / AIF / PDCD8 Antibody (clone 4E7) LS-C169395
LifeSpan Biosciences
catalog: LS-C169395
mouse monoclonal (4E7)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry
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AIFM1 / AIF / PDCD8 Antibody (Tyr85) LS-C176970
LifeSpan Biosciences
catalog: LS-C176970
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of AIF-M1 (Y85) pAb in extracts from HUVEC cells.
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AIFM1 / AIF / PDCD8 Antibody (aa517-531) LS-C178847
LifeSpan Biosciences
catalog: LS-C178847
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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AIFM1 / AIF / PDCD8 Antibody (aa70-98, AP) LS-C275069
LifeSpan Biosciences
catalog: LS-C275069
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa70-98, APC) LS-C275070
LifeSpan Biosciences
catalog: LS-C275070
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa70-98, FITC) LS-C275071
LifeSpan Biosciences
catalog: LS-C275071
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa70-98, Biotin) LS-C275072
LifeSpan Biosciences
catalog: LS-C275072
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa70-98, Azide-free, HRP) LS-C275073
LifeSpan Biosciences
catalog: LS-C275073
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa70-98, PE) LS-C275078
LifeSpan Biosciences
catalog: LS-C275078
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa100-150) LS-C289500
LifeSpan Biosciences
catalog: LS-C289500
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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AIFM1 / AIF / PDCD8 Antibody (aa563-613) LS-C289501
LifeSpan Biosciences
catalog: LS-C289501
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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AIFM1 / AIF / PDCD8 Antibody (aa103-322) LS-C292922
LifeSpan Biosciences
catalog: LS-C292922
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation

Western Blot; Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Heart Tissue; Lane3: Human Hela Cells; Lane4: Human A549 Cells.

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AIFM1 / AIF / PDCD8 Antibody LS-C306162
LifeSpan Biosciences
catalog: LS-C306162
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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AIFM1 / AIF / PDCD8 Antibody LS-C309376
LifeSpan Biosciences
catalog: LS-C309376
domestic rabbit polyclonal
reactivity: human
application: western blot
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AIFM1 / AIF / PDCD8 Antibody (aa596-613) LS-C313335
LifeSpan Biosciences
catalog: LS-C313335
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

AIFM1 / AIF antibody. IHC(P): Rat Cardiac Muscle Tissue.

WB of AIFM1 / AIF antibody. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: K562 Cell Lysate. Lane 4: HEPG2 Cell Lysate. Lane 5: A431 Cell Lysate. Lane 6: NIH3T3 Cell Lysate.
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AIFM1 / AIF / PDCD8 Antibody (aa70-98) LS-C326833
LifeSpan Biosciences
catalog: LS-C326833
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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AIFM1 / AIF / PDCD8 Antibody LS-C332175
LifeSpan Biosciences
catalog: LS-C332175
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human esophageal tissue.

Immunofluorescence analysis of HeLa cells.
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AIFM1 / AIF / PDCD8 Antibody (N-Terminus) LS-C353147
LifeSpan Biosciences
catalog: LS-C353147
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of AIFM1 expression in MCF7 (A); Molt (B); Jurkat (C); mouse liver (D); rat heart (E) whole cell lysates.

Immunohistochemical analysis of AIFM1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of AIFM1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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AIFM1 / AIF / PDCD8 Antibody LS-C356819
LifeSpan Biosciences
catalog: LS-C356819
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-AIFM1 Antibody at 1:1000 dilution Lane 1: K562 whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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AIFM1 / AIF / PDCD8 Antibody (aa582-613) LS-C357554
LifeSpan Biosciences
catalog: LS-C357554
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

AIF antibody Western blot. All lanes: Anti AIF at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Rat Brain Tissue Lysate at 50 ug. Lane 3: Mouse Stomach Tissue Lysate at 50 ug. Lane 4: Mouse Spleen Tissue Lysate at 50 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Lane 6: U87 Whole Cell Lysate at 40 ug. Lane 7: PANC Whole Cell Lysate at 40 ug. Predicted band size: 67 kD. Observed band size: 85 kD.

AIF antibody IHC-paraffin: Mouse Cardiac Muscle Tissue.

AIF antibody ICC: SMMC-7721 Cell.
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AIFM1 / AIF / PDCD8 Antibody LS-C358736
LifeSpan Biosciences
catalog: LS-C358736
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
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AIFM1 / AIF / PDCD8 Antibody (aa103-612, FITC) LS-C369199
LifeSpan Biosciences
catalog: LS-C369199
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa103-612, HRP) LS-C369203
LifeSpan Biosciences
catalog: LS-C369203
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa103-612) LS-C369208
LifeSpan Biosciences
catalog: LS-C369208
domestic rabbit polyclonal
reactivity: human
application: ELISA
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AIFM1 / AIF / PDCD8 Antibody (aa103-612, Biotin) LS-C369210
LifeSpan Biosciences
catalog: LS-C369210
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA
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AIFM1 / AIF / PDCD8 Antibody (30-110 aa, N-terminal) LS-C381966
LifeSpan Biosciences
catalog: LS-C381966
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of AIF-M1 antibody
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