IHC-plus™ DOK2 Antibody (C-Terminus) LS-B1520
LifeSpan Biosciences
catalog: LS-B1520

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-DOK2 antibody IHC of human liver, Kupffer cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DOK2 Antibody (aa266-315) LS-C118093
LifeSpan Biosciences
catalog: LS-C118093

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using p56 Dok-2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205, using p56 Dok-2 Antibody. The lane on the right is blocked with the synthesized peptide.
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DOK2 Antibody (phospho-Tyr299) LS-C123410
LifeSpan Biosciences
catalog: LS-C123410

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry

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DOK2 Antibody (C-Terminus) LS-C6435
LifeSpan Biosciences
catalog: LS-C6435

domestic rabbit polyclonal
reactivity: human
application: western blot

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DOK2 Antibody (aa393-412) LS-C22888
LifeSpan Biosciences
catalog: LS-C22888

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

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DOK2 Antibody (aa380-412) LS-C99154
LifeSpan Biosciences
catalog: LS-C99154

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of DOK2 (arrow) using DOK2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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DOK2 Antibody (phospho-Tyr299) LS-C117334
LifeSpan Biosciences
catalog: LS-C117334

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ml 15', Jurkat cells treated with insulin 0.01U/ml 15' and 293 cells treated with serum 20% 15', using p56 Dok-2 (Phospho-Tyr299) Antibody. The lane on the right is blocked with the phospho peptide.
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DOK2 Antibody LS-C123411
LifeSpan Biosciences
catalog: LS-C123411

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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DOK2 Antibody (clone OTI2A3) LS-C173097
LifeSpan Biosciences
catalog: LS-C173097

mouse monoclonal (OTI2A3)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2.

Anti-DOK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK2 antibody, and then analyzed by flow cytometry.
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DOK2 Antibody (clone OTI1G9) LS-C173098
LifeSpan Biosciences
catalog: LS-C173098

mouse monoclonal (OTI1G9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2.
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DOK2 Antibody (clone OTI3D6) LS-C173201
LifeSpan Biosciences
catalog: LS-C173201

mouse monoclonal (OTI3D6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2.

Anti-DOK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK2 antibody, and then analyzed by flow cytometry.
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DOK2 Antibody (Arg293) LS-C177109
LifeSpan Biosciences
catalog: LS-C177109

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of Dok-2 (R293) pAb in extracts from COLO205 cells.
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DOK2 Antibody (phospho-Tyr299) LS-C178049
LifeSpan Biosciences
catalog: LS-C178049

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunocytochemistry

Western blot of p-Dok-2 (Y299) pAb in extracts from 293 serum cells.
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DOK2 Antibody (aa292-341) LS-C200452
LifeSpan Biosciences
catalog: LS-C200452

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of the lysates from HeLa cells using p56 Dok-2 antibody.
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DOK2 Antibody (aa380-412, AP) LS-C244211
LifeSpan Biosciences
catalog: LS-C244211

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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DOK2 Antibody (aa380-412, APC) LS-C244212
LifeSpan Biosciences
catalog: LS-C244212

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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DOK2 Antibody (aa380-412, Biotin) LS-C244213
LifeSpan Biosciences
catalog: LS-C244213

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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DOK2 Antibody (aa380-412, FITC) LS-C244214
LifeSpan Biosciences
catalog: LS-C244214

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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DOK2 Antibody (aa380-412, PE) LS-C244217
LifeSpan Biosciences
catalog: LS-C244217

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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DOK2 Antibody (aa380-412, Azide-free, HRP) LS-C247546
LifeSpan Biosciences
catalog: LS-C247546

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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DOK2 Antibody (phospho-Tyr299) LS-C335903
LifeSpan Biosciences
catalog: LS-C335903

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of extracts from Jurkat cells.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.

Immunofluorescence staining of methanol-fixed Hela cells.
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DOK2 Antibody (clone OTI1A4) LS-C337416
LifeSpan Biosciences
catalog: LS-C337416

mouse monoclonal (OTI1A4)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2.

Anti-DOK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2.
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DOK2 Antibody (clone OTI2D10) LS-C337459
LifeSpan Biosciences
catalog: LS-C337459

mouse monoclonal (OTI2D10)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2.
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DOK2 Antibody (phospho-Tyr299) LS-C353120
LifeSpan Biosciences
catalog: LS-C353120

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of DOK2 (pY299) expression in A549 Insulin-treated (A); HepG2 (B) whole cell lysates.

Immunohistochemical analysis of DOK2 (pY299) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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DOK2 Antibody (240-320 aa) LS-C380677
LifeSpan Biosciences
catalog: LS-C380677

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunocytochemistry

Western blot of Phospho-Dok-2 (Y299) antibody
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DOK2 Antibody (290-370 aa) LS-C381703
LifeSpan Biosciences
catalog: LS-C381703

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: ELISA, immunohistochemistry

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DOK2 Antibody (290-370 aa) LS-C383181
LifeSpan Biosciences
catalog: LS-C383181

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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DOK2 Antibody (240-320 aa) LS-C383184
LifeSpan Biosciences
catalog: LS-C383184

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Dok-2 antibody
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