IHC-plus™ CCNH / Cyclin H Antibody (clone 1B8) LS-B6020
LifeSpan Biosciences
catalog: LS-B6020
mouse monoclonal (1B8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of CCNH expression in transfected 293T cell line. Lane1: CCNH transfected lysate (37.6 KDa). Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CCNH is approximately 1 ng/ml as a capture antibody.
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CCNH / Cyclin H Antibody (aa274-323) LS-C117869
LifeSpan Biosciences
catalog: LS-C117869
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells, using Cyclin H Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cyclin H Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2, LOVO, and RAW264.7 cells, using Cyclin H Antibody. The lane on the right is blocked with the synthesized peptide.
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CCNH / Cyclin H Antibody LS-C331195
LifeSpan Biosciences
catalog: LS-C331195
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
citations: 1

Western blot analysis of extracts of various cell lines.

Immunofluorescence analysis of HeLa cells.

Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug CCNH antibody. Western blot was performed from the immunoprecipitate using CCNH antibodyat a dilition of 1:1000.
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CCNH / Cyclin H Antibody (C-Terminus) LS-C30772
LifeSpan Biosciences
catalog: LS-C30772
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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CCNH / Cyclin H Antibody LS-C64121
LifeSpan Biosciences
catalog: LS-C64121
mouse monoclonal
reactivity: human
application: western blot
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CCNH / Cyclin H Antibody (C-Terminus) LS-C102865
LifeSpan Biosciences
catalog: LS-C102865
domestic rabbit polyclonal
reactivity: human
application: western blot
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CCNH / Cyclin H Antibody (aa50-99) LS-C116966
LifeSpan Biosciences
catalog: LS-C116966
domestic rabbit polyclonal
reactivity: human
application: western blot
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CCNH / Cyclin H Antibody (aa269-299) LS-C168475
LifeSpan Biosciences
catalog: LS-C168475
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).

CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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CCNH / Cyclin H Antibody (aa24-52) LS-C168476
LifeSpan Biosciences
catalog: LS-C168476
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

CCNH Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).

CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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CCNH / Cyclin H Antibody (Glu311) LS-C176992
LifeSpan Biosciences
catalog: LS-C176992
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Western blot of Cyclin H (E311) pAb in extracts from K562 cells.
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CCNH / Cyclin H Antibody LS-C178271
LifeSpan Biosciences
catalog: LS-C178271
mouse monoclonal
reactivity: human
application: western blot

Western blot detection of CCNH in Jurkat,K562&A431 cell lysates using CCNH antibody (1:1000 diluted).
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CCNH / Cyclin H Antibody (clone 2125C2a) LS-C184681
LifeSpan Biosciences
catalog: LS-C184681
mouse monoclonal (2125C2a)
reactivity: human
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CCNH / Cyclin H Antibody LS-C22576
LifeSpan Biosciences
catalog: LS-C22576
mouse monoclonal
reactivity: human
application: western blot, ELISA
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CCNH / Cyclin H Antibody (phospho-Thr315) LS-C198867
LifeSpan Biosciences
catalog: LS-C198867
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot

Western blot analysis of lysates from HepG2 cells, Jurkat cells, HUVEC cells and A549 cells, using Cyclin H (Phospho-Thr315) Antibody. The lane on the right is blocked with the phospho peptide.
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CCNH / Cyclin H Antibody (aa269-299, AP) LS-C208261
LifeSpan Biosciences
catalog: LS-C208261
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, flow cytometry
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CCNH / Cyclin H Antibody (aa269-299, APC) LS-C208262
LifeSpan Biosciences
catalog: LS-C208262
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, flow cytometry
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CCNH / Cyclin H Antibody (aa269-299, Biotin) LS-C208263
LifeSpan Biosciences
catalog: LS-C208263
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, flow cytometry
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CCNH / Cyclin H Antibody (aa269-299, FITC) LS-C208264
LifeSpan Biosciences
catalog: LS-C208264
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, flow cytometry
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CCNH / Cyclin H Antibody (aa269-299, Azide-free, HRP) LS-C208265
LifeSpan Biosciences
catalog: LS-C208265
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, flow cytometry
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CCNH / Cyclin H Antibody (aa269-299, PE) LS-C208266
LifeSpan Biosciences
catalog: LS-C208266
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, flow cytometry
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CCNH / Cyclin H Antibody LS-C306789
LifeSpan Biosciences
catalog: LS-C306789
mouse polyclonal
reactivity: human
application: western blot, immunocytochemistry
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CCNH / Cyclin H Antibody LS-C192320
LifeSpan Biosciences
catalog: LS-C192320
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of CCNH pAb in extracts from Jurkat, K562, SW620, HT29 and MCF7 cells.
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CCNH / Cyclin H Antibody (aa269-299) LS-C320129
LifeSpan Biosciences
catalog: LS-C320129
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, flow cytometry
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IHC-plus™ CCNH / Cyclin H Antibody (aa262-311) LS-B12480
LifeSpan Biosciences
catalog: LS-B12480
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of the lysates from HepG2 cells using Cyclin H antibody.
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CCNH / Cyclin H Antibody (C-Terminus) LS-C358931
LifeSpan Biosciences
catalog: LS-C358931
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot analysis of Cyclin H (pT315) expression in Jurkat UV-treated (A); A549 UV-treated (B) whole cell lysates.

Immunohistochemical analysis of Cyclin H (pT315) staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CCNH / Cyclin H Antibody (C-Terminus) LS-C358933
LifeSpan Biosciences
catalog: LS-C358933
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of Cyclin H expression in HeLa (A); LOVO (B); RAW264.7 (C) whole cell lysates.

Immunohistochemical analysis of Cyclin H staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of Cyclin H staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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CCNH / Cyclin H Antibody (250-330 aa) LS-C380948
LifeSpan Biosciences
catalog: LS-C380948
domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
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CCNH / Cyclin H Antibody (250-330 aa) LS-C382971
LifeSpan Biosciences
catalog: LS-C382971
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

Western blot of Cyclin H antibody
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