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CCNB1 / Cyclin B1 Antibody (phospho-Ser147) LS-C22491
LifeSpan Biosciences
catalog: LS-C22491
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser126) LS-C18812
LifeSpan Biosciences
catalog: LS-C18812
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
ELISA
,
immunoprecipitation
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C22492
LifeSpan Biosciences
catalog: LS-C22492
mouse monoclonal
reactivity:
human
application:
western blot
,
immunoprecipitation
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C22493
LifeSpan Biosciences
catalog: LS-C22493
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
,
immunoprecipitation
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (C-Terminus) LS-C40284
LifeSpan Biosciences
catalog: LS-C40284
domestic rabbit polyclonal
reactivity:
human
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C64076
LifeSpan Biosciences
catalog: LS-C64076
mouse monoclonal
reactivity:
human
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (N-Terminus) LS-C64077
LifeSpan Biosciences
catalog: LS-C64077
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunocytochemistry
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser133) LS-C64078
LifeSpan Biosciences
catalog: LS-C64078
domestic rabbit monoclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunoprecipitation
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone V152, Azide-free) LS-C88094
LifeSpan Biosciences
catalog: LS-C88094
mouse monoclonal (V152)
reactivity:
human
application:
western blot
,
immunohistochemistry
,
flow cytometry
,
immunohistochemistry - paraffin section
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone GNS1) LS-C88533
LifeSpan Biosciences
catalog: LS-C88533
mouse monoclonal (GNS1)
reactivity:
human
application:
western blot
,
immunocytochemistry
,
immunoprecipitation
,
flow cytometry
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone GNS11, Biotin) LS-C88536
LifeSpan Biosciences
catalog: LS-C88536
mouse monoclonal (GNS11)
reactivity:
human
conjugate: biotin
application:
immunohistochemistry
,
immunocytochemistry
,
immunoprecipitation
,
immunohistochemistry - paraffin section
Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of lymphoid cells. This image was taken for the unmodified form of this product. Other forms have not been tested.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone GNS11, Azide-free) LS-C88537
LifeSpan Biosciences
catalog: LS-C88537
mouse monoclonal (GNS11)
reactivity:
human
application:
immunohistochemistry
,
immunocytochemistry
,
immunoprecipitation
,
immunohistochemistry - paraffin section
Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of lymphoid cells. This image was taken for the unmodified form of this product. Other forms have not been tested.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126) LS-C100240
LifeSpan Biosciences
catalog: LS-C100240
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Western blot of anti-Cyclin B1 Antibody (S126) in A375 cell line lysates (35 ug/lane). Cyclin B1(arrow) was detected using the purified antibody.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (C-Terminus) LS-C110801
LifeSpan Biosciences
catalog: LS-C110801
domestic rabbit polyclonal
reactivity:
human
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa91-140) LS-C117561
LifeSpan Biosciences
catalog: LS-C117561
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Immunofluorescence analysis of HeLa cells, using Cyclin B1 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Cyclin B1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, treated with serum 10% 15', using Cyclin B1 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C18811
LifeSpan Biosciences
catalog: LS-C18811
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
,
immunoprecipitation
,
immunohistochemistry - paraffin section
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser147) LS-C123353
LifeSpan Biosciences
catalog: LS-C123353
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
immunohistochemistry
,
immunocytochemistry
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone 1C8) LS-C133019
LifeSpan Biosciences
catalog: LS-C133019
mouse monoclonal (1C8)
reactivity:
human
application:
western blot
,
ELISA
Western blot of CCNB1 expression in JAR cell lysate.
Detection limit for recombinant GST tagged CCNB1 is approximately 0.1 ng/ml as a capture antibody.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (N-Terminus) LS-C137140
LifeSpan Biosciences
catalog: LS-C137140
domestic rabbit polyclonal
reactivity:
human
application:
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
IHC-plus™ CCNB1 / Cyclin B1 Antibody (clone V152) LS-B8608
LifeSpan Biosciences
catalog: LS-B8608
mouse monoclonal (V152)
reactivity:
human
application:
western blot
,
immunohistochemistry
,
flow cytometry
,
immunohistochemistry - paraffin section
Anti-Cyclin B1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35) LS-C157610
LifeSpan Biosciences
catalog: LS-C157610
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
Western blot of lysate from HeLa cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody or CCNB1-S9 Antibody.Lysate at 15ug per lane.Antibody was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Dot blot of CCNB1 Antibody (Phospho S35) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa381-411) LS-C168461
LifeSpan Biosciences
catalog: LS-C168461
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
CCNB1 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The CCNB1 antibody detected the CCNB1 protein (arrow).
CCNB1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human uterus tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa241-268) LS-C168462
LifeSpan Biosciences
catalog: LS-C168462
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
CCNB1 Antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The CCNB1 antibody detected the CCNB1 protein (arrow).
CCNB1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human uterus tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone 1B10) LS-C169266
LifeSpan Biosciences
catalog: LS-C169266
mouse monoclonal (1B10)
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
,
immunohistochemistry - paraffin section
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone 5G6) LS-C169274
LifeSpan Biosciences
catalog: LS-C169274
mouse monoclonal (5G6)
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
,
immunocytochemistry
,
flow cytometry
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ile120) LS-C176271
LifeSpan Biosciences
catalog: LS-C176271
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Western blot of Cyclin B1 (I120) pAb in extracts from HeLa cells treated with serum 10% 15' HepG2.
IHC of Cyclin B1 (I120) pAb in paraffin-embedded human breast carcinoma tissue.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser126) LS-C177972
LifeSpan Biosciences
catalog: LS-C177972
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunohistochemistry
Western blot of P-Cyclin B1 (S126) pAb in extracts from raw264.7 cells.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone V152) LS-C179650
LifeSpan Biosciences
catalog: LS-C179650
mouse monoclonal (V152)
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone V92.1) LS-C179693
LifeSpan Biosciences
catalog: LS-C179693
mouse monoclonal (V92.1)
reactivity:
human
application:
immunoprecipitation
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone 2113C1a) LS-C184652
LifeSpan Biosciences
catalog: LS-C184652
mouse monoclonal (2113C1a)
reactivity:
human
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C189708
LifeSpan Biosciences
catalog: LS-C189708
mouse monoclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa121-170) LS-C198856
LifeSpan Biosciences
catalog: LS-C198856
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Western blot analysis of lysates from Jurkat cells, treated with UV 15', using Cyclin B1 Antibody. The lane on the right is blocked with the synthesized peptide.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser126) LS-C198857
LifeSpan Biosciences
catalog: LS-C198857
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin B1 (Phospho-Ser126) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 15', using Cyclin B1 (Phospho-Ser126) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser147) LS-C198858
LifeSpan Biosciences
catalog: LS-C198858
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunohistochemistry
,
immunohistochemistry - paraffin section
Immunohistochemistry analysis of paraffin-embedded human placenta, using Cyclin B1 (Phospho-Ser147) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells treated with UV 15', using Cyclin B1 (Phospho-Ser147) Antibody. The lane on the right is blocked with the phospho peptide.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa1-30) LS-C203885
LifeSpan Biosciences
catalog: LS-C203885
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Western blot of CCNB1 Antibody (N-term S9) antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. CCNB1 Antibody(arrow) was detected using the purified antibody.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa106-133) LS-C203886
LifeSpan Biosciences
catalog: LS-C203886
domestic rabbit polyclonal
reactivity:
human
application:
western blot
CCNB1 Antibody (Center S126/S128) western blot of U251 cell line lysates (35 ug/lane). The CCNB1 antibody detected the CCNB1 protein (arrow).
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa155-182) LS-C203887
LifeSpan Biosciences
catalog: LS-C203887
domestic rabbit polyclonal
reactivity:
human
application:
western blot
CCNB1 Antibody (Center Y177) western blot of NCI-H460 cell line lysates (35 ug/lane). The CCNB1 antibody detected the CCNB1 protein (arrow).
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa299-326) LS-C203888
LifeSpan Biosciences
catalog: LS-C203888
domestic rabbit polyclonal
reactivity:
human
application:
western blot
CCNB1 Antibody (C-term T321) western blot of K562 cell line lysates (35 ug/lane). The CCNB1 antibody detected the CCNB1 protein (arrow).
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa103-130) LS-C203981
LifeSpan Biosciences
catalog: LS-C203981
domestic rabbit polyclonal
reactivity:
human
,
mouse
application:
western blot
Mouse CCNB1 Antibody (Center S123/S125)western blot of mouse NIH/3T3 cell line and mouse spleen tissue lysates (35 ug/lane). The mouse CCNB1 antibody detected the mouse CCNB1 protein (arrow).
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35, AP) LS-C213116
LifeSpan Biosciences
catalog: LS-C213116
domestic rabbit polyclonal
reactivity:
human
conjugate: AP
modification: phosphorylated
application:
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35, APC) LS-C213117
LifeSpan Biosciences
catalog: LS-C213117
domestic rabbit polyclonal
reactivity:
human
conjugate: APC
modification: phosphorylated
application:
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35, Biotin) LS-C213118
LifeSpan Biosciences
catalog: LS-C213118
domestic rabbit polyclonal
reactivity:
human
conjugate: biotin
modification: phosphorylated
application:
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35, Azide-free, HRP) LS-C213119
LifeSpan Biosciences
catalog: LS-C213119
domestic rabbit polyclonal
reactivity:
human
conjugate: HRP
modification: phosphorylated
application:
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35, FITC) LS-C213120
LifeSpan Biosciences
catalog: LS-C213120
domestic rabbit polyclonal
reactivity:
human
conjugate: FITC
modification: phosphorylated
application:
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35, PE) LS-C213121
LifeSpan Biosciences
catalog: LS-C213121
domestic rabbit polyclonal
reactivity:
human
conjugate: PE
modification: phosphorylated
application:
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126, AP) LS-C218872
LifeSpan Biosciences
catalog: LS-C218872
domestic rabbit polyclonal
reactivity:
human
conjugate: AP
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126, APC) LS-C218873
LifeSpan Biosciences
catalog: LS-C218873
domestic rabbit polyclonal
reactivity:
human
conjugate: APC
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126, Biotin) LS-C218874
LifeSpan Biosciences
catalog: LS-C218874
domestic rabbit polyclonal
reactivity:
human
conjugate: biotin
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126, FITC) LS-C218875
LifeSpan Biosciences
catalog: LS-C218875
domestic rabbit polyclonal
reactivity:
human
conjugate: FITC
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126, Azide-free, HRP) LS-C218876
LifeSpan Biosciences
catalog: LS-C218876
domestic rabbit polyclonal
reactivity:
human
conjugate: HRP
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126, PE) LS-C218877
LifeSpan Biosciences
catalog: LS-C218877
domestic rabbit polyclonal
reactivity:
human
conjugate: PE
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C310055
LifeSpan Biosciences
catalog: LS-C310055
domestic rabbit polyclonal
reactivity:
human
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone RBT-B1) LS-C312017
LifeSpan Biosciences
catalog: LS-C312017
domestic rabbit recombinant (RBT-B1)
reactivity:
human
application:
immunohistochemistry
,
immunohistochemistry - paraffin section
,
immunohistochemistry - frozen section
IHC of Cyclin B1 on FFPE Cervical Cancer tissue Intended Use For In Vitro Diagnostic Use.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C193043
LifeSpan Biosciences
catalog: LS-C193043
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
Western blot of extracts of HepG2 cell lines, using CCNB1 antibody.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser35) LS-C320103
LifeSpan Biosciences
catalog: LS-C320103
domestic rabbit polyclonal
reactivity:
human
application:
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Ser126) LS-C327148
LifeSpan Biosciences
catalog: LS-C327148
domestic rabbit polyclonal
reactivity:
human
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C330940
LifeSpan Biosciences
catalog: LS-C330940
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Western blot analysis of extracts of HepG2 cells, using CCNB1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C331859
LifeSpan Biosciences
catalog: LS-C331859
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunoprecipitation
Western blot analysis of extracts of various cell lines.
Immunofluorescence analysis of A549 cells.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser147) LS-C335941
LifeSpan Biosciences
catalog: LS-C335941
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
Western blot analysis of extracts from MDA cells.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
Immunofluorescence staining of methanol-fixed HeLa cells.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa1-433) LS-C343971
LifeSpan Biosciences
catalog: LS-C343971
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Cyclin B1 antibody Western blot. All lanes: Anti Cyclin B1 at 0.5 ug/ml. WB: Recombinant Human Cyclin B1 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone RBT-B1, Ready-to-Use) LS-C348413
LifeSpan Biosciences
catalog: LS-C348413
domestic rabbit recombinant (RBT-B1)
reactivity:
human
application:
immunohistochemistry - paraffin section
,
immunohistochemistry - frozen section
IHC of Cyclin B1 on FFPE Cervical Cancer tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser126) LS-C351951
LifeSpan Biosciences
catalog: LS-C351951
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunohistochemistry
,
immunoprecipitation
,
immunohistochemistry - paraffin section
Western blot analysis of Cyclin B1 (pS126) expression in HT29 nocodazole-treated (A); Jurkat (B); HeLa (C); Raw264.7 (D) whole cell lysates.
Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (Internal) LS-C351953
LifeSpan Biosciences
catalog: LS-C351953
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Western blot analysis of Cyclin B1 expression in Jurkat (A); K562 (B); HEK293T (C); HeLa (D) whole cell lysates.
Immunohistochemical analysis of Cyclin B1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Cyclin B1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (clone V92.1) LS-C357828
LifeSpan Biosciences
catalog: LS-C357828
mouse monoclonal (V92.1)
reactivity:
human
application:
immunocytochemistry
,
immunoprecipitation
,
flow cytometry
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C357830
LifeSpan Biosciences
catalog: LS-C357830
domestic rabbit polyclonal
reactivity:
human
application:
western blot
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (phospho-Ser147) LS-C358934
LifeSpan Biosciences
catalog: LS-C358934
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Western blot analysis of Cyclin B1 (pS147) expression in MDA anisomycin-treated (A); HeLa UV-treated (B) whole cell lysates.
Immunohistochemical analysis of Cyclin B1 (pS147) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Cyclin B1 (pS147) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (aa239-433) LS-C373128
LifeSpan Biosciences
catalog: LS-C373128
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunocytochemistry
,
immunoprecipitation
Western Blot; Sample: Recombinant CCNB, Human.
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (60-140 aa) LS-C380676
LifeSpan Biosciences
catalog: LS-C380676
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
ELISA
,
immunohistochemistry
Western blot of Phospho-Cyclin B1 (S126) antibody
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (90-170 aa) LS-C381288
LifeSpan Biosciences
catalog: LS-C381288
domestic rabbit polyclonal
reactivity:
human
modification: phosphorylated
application:
western blot
,
ELISA
,
immunohistochemistry
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (60-140 aa) LS-C382972
LifeSpan Biosciences
catalog: LS-C382972
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
,
immunocytochemistry
Western blot of Cyclin B1 antibody
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody (90-170 aa) LS-C382973
LifeSpan Biosciences
catalog: LS-C382973
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
ELISA
quantity:
price:
to the supplier
CCNB1 / Cyclin B1 Antibody LS-C387863
LifeSpan Biosciences
catalog: LS-C387863
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunohistochemistry
quantity:
price:
to the supplier