IHC-plus™ ACTN3 Antibody (aa1-50) LS-B4907
LifeSpan Biosciences
catalog: LS-B4907
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACTN3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot analysis of lysates from HT-29 cells, using ACTN3 Antibody. The lane on the right is blocked with the synthesized peptide.
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IHC-plus™ ACTN3 Antibody (aa180-229) LS-B5732
LifeSpan Biosciences
catalog: LS-B5732
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACTN3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ACTN3 Antibody (Glu25) LS-C176865
LifeSpan Biosciences
catalog: LS-C176865
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot of Actinin-3 (E25) pAb in extracts from PC12 cells.
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IHC-plus™ ACTN3 Antibody (aa409-437) LS-B9666
LifeSpan Biosciences
catalog: LS-B9666
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-ACTN3 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

ACTN3 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The ACTN3 antibody detected the ACTN3 protein (arrow).
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ACTN3 Antibody (aa409-437, AP) LS-C250773
LifeSpan Biosciences
catalog: LS-C250773
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry
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ACTN3 Antibody (aa409-437, APC) LS-C250774
LifeSpan Biosciences
catalog: LS-C250774
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry
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ACTN3 Antibody (aa409-437, Biotin) LS-C250775
LifeSpan Biosciences
catalog: LS-C250775
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry
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ACTN3 Antibody (aa409-437, FITC) LS-C250776
LifeSpan Biosciences
catalog: LS-C250776
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry
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ACTN3 Antibody (aa409-437, Azide-free, HRP) LS-C250777
LifeSpan Biosciences
catalog: LS-C250777
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry
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ACTN3 Antibody (aa409-437, PE) LS-C250778
LifeSpan Biosciences
catalog: LS-C250778
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry
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ACTN3 Antibody (aa162-416) LS-C314695
LifeSpan Biosciences
catalog: LS-C314695
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of recombinant ACTN3.

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ACTN3 Antibody (aa409-437) LS-C319315
LifeSpan Biosciences
catalog: LS-C319315
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry
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ACTN3 Antibody (N-Terminus) LS-C351785
LifeSpan Biosciences
catalog: LS-C351785
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of ACTN3 expression in HeLa (A); mouse muscle (B); rat muscle (C) whole cell lysates.

Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ACTN3 Antibody LS-C356809
LifeSpan Biosciences
catalog: LS-C356809
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-ACTN3 Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: human skeletal muscle lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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