IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) LS-A3106
LifeSpan Biosciences
catalog: LS-A3106

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-SPHK1 antibody IHC of human adrenal, pheochromocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) LS-A3102
LifeSpan Biosciences
catalog: LS-A3102

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-SPHK1 antibody IHC of human adrenal, zona glomerulosa. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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SPHK / SPHK1 Antibody LS-C26924
LifeSpan Biosciences
catalog: LS-C26924

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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SPHK / SPHK1 Antibody (aa286-315) LS-C100769
LifeSpan Biosciences
catalog: LS-C100769

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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SPHK / SPHK1 Antibody (C-Terminus) LS-C26796
LifeSpan Biosciences
catalog: LS-C26796

domestic rabbit polyclonal
reactivity: human
application: western blot

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SPHK / SPHK1 Antibody (phospho-Ser225) LS-C26925
LifeSpan Biosciences
catalog: LS-C26925

domestic rabbit polyclonal
reactivity: human, mouse
modification: phosphorylated
application: western blot

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SPHK / SPHK1 Antibody (aa176-189) LS-C87246
LifeSpan Biosciences
catalog: LS-C87246

domestic goat polyclonal
reactivity: human
application: western blot, immunoprecipitation

Antibody (2 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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SPHK / SPHK1 Antibody (aa1-30) LS-C100768
LifeSpan Biosciences
catalog: LS-C100768

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysate from A431 cell line, using SPHK1 Antibody (M1). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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SPHK / SPHK1 Antibody (aa59-89) LS-C100770
LifeSpan Biosciences
catalog: LS-C100770

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemical of paraffin-embedded H. kidney section using SPHK1 Antibody(N-term P74). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot of lysates from HepG2 cell line and human heart, skeletal muscle, kidney tissue lysate(from left to right), using SPHK1 Antibody (N-term P74). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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SPHK / SPHK1 Antibody LS-C27023
LifeSpan Biosciences
catalog: LS-C27023

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

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SPHK / SPHK1 Antibody LS-C140528
LifeSpan Biosciences
catalog: LS-C140528

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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SPHK / SPHK1 Antibody (aa334-384) LS-C142727
LifeSpan Biosciences
catalog: LS-C142727

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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SPHK / SPHK1 Antibody (aa176-225) LS-C146131
LifeSpan Biosciences
catalog: LS-C146131

domestic rabbit polyclonal
reactivity: human
application: western blot

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SPHK / SPHK1 Antibody (FITC) LS-C146698
LifeSpan Biosciences
catalog: LS-C146698

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, flow cytometry

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SPHK / SPHK1 Antibody LS-C147008
LifeSpan Biosciences
catalog: LS-C147008

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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IHC-plus™ SPHK / SPHK1 Antibody LS-B6973
LifeSpan Biosciences
catalog: LS-B6973

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Anti-SPHK1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ SPHK / SPHK1 Antibody (N-Terminus) LS-A3104
LifeSpan Biosciences
catalog: LS-A3104

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Anti-SPHK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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SPHK / SPHK1 Antibody (aa313-343) LS-C161448
LifeSpan Biosciences
catalog: LS-C161448

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (C-term R414). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.

SPHK1 Antibody (C-term R414)IHC of formalin-fixed and paraffin-embedded skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry.

SPHK1 Antibody (C-term R414) flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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SPHK / SPHK1 Antibody (aa286-315, APC) LS-C270187
LifeSpan Biosciences
catalog: LS-C270187

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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SPHK / SPHK1 Antibody (aa286-315, PE) LS-C270189
LifeSpan Biosciences
catalog: LS-C270189

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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SPHK / SPHK1 Antibody (aa286-315, AP) LS-C270190
LifeSpan Biosciences
catalog: LS-C270190

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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SPHK / SPHK1 Antibody (aa1-30, APC) LS-C282962
LifeSpan Biosciences
catalog: LS-C282962

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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SPHK / SPHK1 Antibody (aa1-30, AP) LS-C282963
LifeSpan Biosciences
catalog: LS-C282963

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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SPHK / SPHK1 Antibody (aa1-30, PE) LS-C282965
LifeSpan Biosciences
catalog: LS-C282965

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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SPHK / SPHK1 Antibody (aa334-384) LS-C288816
LifeSpan Biosciences
catalog: LS-C288816

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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SPHK / SPHK1 Antibody (aa1-50) LS-C288931
LifeSpan Biosciences
catalog: LS-C288931

domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation

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SPHK / SPHK1 Antibody LS-C192442
LifeSpan Biosciences
catalog: LS-C192442

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of SPHK1 pAb in extracts from SW640, Hela cells and mouse kidney tissue.
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SPHK / SPHK1 Antibody (aa286-315) LS-C325332
LifeSpan Biosciences
catalog: LS-C325332

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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SPHK / SPHK1 Antibody (aa1-30) LS-C328731
LifeSpan Biosciences
catalog: LS-C328731

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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SPHK / SPHK1 Antibody LS-C330808
LifeSpan Biosciences
catalog: LS-C330808

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

Immunofluorescence analysis of HeLa cells.

Western blot analysis of extracts of various cell lines, using SPHK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunohistochemistry of paraffin-embedded human gastric cancer using SPHK1 Antibodyat dilution of 1:200 (40x lens).
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SPHK / SPHK1 Antibody (clone OTI1A6) LS-C338427
LifeSpan Biosciences
catalog: LS-C338427

mouse monoclonal (OTI1A6)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of extracts (35ug) from 9 different cell lines by using anti-SPHK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPHK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-SPHK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPHK1.
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