LifeSpan Biosciences FLIP antibody

IHC-plus™ CFLAR / FLIP Antibody (aa206-222) LS-B2024
LifeSpan Biosciences
catalog: LS-B2024

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-CFLAR / FLIP antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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FLIP Gamma/Delta Antibody (C-Terminus) LS-C129109
LifeSpan Biosciences
catalog: LS-C129109

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - frozen section

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CFLAR / FLIP Antibody LS-C274
LifeSpan Biosciences
catalog: LS-C274

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 ug/ml.

Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 ug/ml.
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IHC-plus™ CFLAR / FLIP Antibody LS-B3187
LifeSpan Biosciences
catalog: LS-B3187

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot of FLIPg/d in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIPg/d antibody at 1:1000 dilution.

Immunocytochemical staining of HeLa cells using FLIPg/d antibody at 2 µg/ml.

Anti-CFLAR / FLIP antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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CFLAR / FLIP Antibody LS-C272
LifeSpan Biosciences
catalog: LS-C272

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry

Western blot of mFLIP in NIH/3T3 whole cell lysate with anti-mFLIP (CT) at 1:500 dilution.

Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 ug/ml.
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CFLAR / FLIP Antibody LS-C275
LifeSpan Biosciences
catalog: LS-C275

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry

Western blot of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 0.5 ug/ml.

Immunocytochemistry of FLIPa in Jurkat cells with FLIPa antibody at 10 ug/ml.

Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 10 ug/ml.
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CFLAR / FLIP Antibody (C-Terminus) LS-C15228
LifeSpan Biosciences
catalog: LS-C15228

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

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CFLAR / FLIP Antibody (C-Terminus) LS-C15234
LifeSpan Biosciences
catalog: LS-C15234

domestic rabbit polyclonal
reactivity: human
application: western blot

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CFLAR / FLIP Antibody (N-Terminus) LS-C15235
LifeSpan Biosciences
catalog: LS-C15235

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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CFLAR / FLIP Antibody (C-Terminus) LS-C48168
LifeSpan Biosciences
catalog: LS-C48168

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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CFLAR / FLIP Antibody (aa1-376, clone 5D8) LS-C93734
LifeSpan Biosciences
catalog: LS-C93734

mouse monoclonal (5D8)
reactivity: human
application: western blot, ELISA

Cell lysates of mouse kidney, MCF7 and rat spleen (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FLIP (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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CFLAR / FLIP Antibody (aa180-229) LS-C112278
LifeSpan Biosciences
catalog: LS-C112278

domestic rabbit polyclonal
reactivity: human
application: western blot

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CFLAR / FLIP Antibody (aa456-471) LS-C121827
LifeSpan Biosciences
catalog: LS-C121827

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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CFLAR / FLIP Antibody (C-Terminus) LS-C123564
LifeSpan Biosciences
catalog: LS-C123564

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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CFLAR / FLIP Antibody (aa1-480) LS-C127264
LifeSpan Biosciences
catalog: LS-C127264

domestic rabbit polyclonal
reactivity: human
application: western blot

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IHC-plus™ CFLAR / FLIP Antibody (clone 2F12) LS-B5753
LifeSpan Biosciences
catalog: LS-B5753

mouse monoclonal (2F12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

CFLAR monoclonal antibody, clone 2F12 Western blot of CFLAR expression in IMR-32.

Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. [antibody concentration 10 ug/ml]

Detection limit for recombinant GST tagged CFLAR is approximately 0.3 ng/ml as a capture antibody.
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CFLAR / FLIP Antibody (aa75-92) LS-C148270
LifeSpan Biosciences
catalog: LS-C148270

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

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CFLAR / FLIP Antibody (aa191-209) LS-C153023
LifeSpan Biosciences
catalog: LS-C153023

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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CFLAR / FLIP Antibody LS-C153028
LifeSpan Biosciences
catalog: LS-C153028

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

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CFLAR / FLIP Antibody (aa449-465) LS-C153029
LifeSpan Biosciences
catalog: LS-C153029

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

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CFLAR / FLIP Antibody (aa145-174) LS-C168708
LifeSpan Biosciences
catalog: LS-C168708

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of CFLAR Antibody in HepG2 cell line lysates (35 ug/lane). CFLAR (arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CFLAR Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

CFLAR Antibody flow cytometry of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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CFLAR / FLIP Antibody (His204) LS-C177054
LifeSpan Biosciences
catalog: LS-C177054

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

IHC of c-FLIP (H204) pAb in paraffin-embedded human colon carcinoma tissue.
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CFLAR / FLIP Antibody (aa100-251, clone 7A3D12) LS-C191863
LifeSpan Biosciences
catalog: LS-C191863

mouse monoclonal (7A3D12)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry

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CFLAR / FLIP Antibody (aa100-251, clone 6G11A6) LS-C191869
LifeSpan Biosciences
catalog: LS-C191869

mouse monoclonal (6G11A6)
reactivity: human
application: western blot, ELISA, immunohistochemistry

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CFLAR / FLIP Antibody (aa181-230) LS-C291276
LifeSpan Biosciences
catalog: LS-C291276

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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CFLAR / FLIP Antibody (aa210-460) LS-C293194
LifeSpan Biosciences
catalog: LS-C293194

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of recombinant CFLAR / FLIP. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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CFLAR / FLIP Antibody LS-C306923
LifeSpan Biosciences
catalog: LS-C306923

mouse polyclonal
reactivity: human
application: western blot

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CFLAR / FLIP Antibody LS-C309900
LifeSpan Biosciences
catalog: LS-C309900

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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CFLAR / FLIP Antibody LS-C332165
LifeSpan Biosciences
catalog: LS-C332165

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot analysis of extracts of various tissues.

Immunohistochemistry of paraffin-embedded human thyroid cancer tissue.
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CFLAR / FLIP Antibody (aa1-272, clone OTI2D3) LS-C339203
LifeSpan Biosciences
catalog: LS-C339203

mouse monoclonal (OTI2D3)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFLAR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CFLAR.
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CFLAR / FLIP Antibody (aa1-272, clone OTI2H3) LS-C339206
LifeSpan Biosciences
catalog: LS-C339206

mouse monoclonal (OTI2H3)
reactivity: human
application: western blot

CFLAR / FLIP Antibody (aa1-272, clone OTI2B9) LS-C339227
LifeSpan Biosciences
catalog: LS-C339227

mouse monoclonal (OTI2B9)
reactivity: human
application: western blot

CFLAR / FLIP Antibody (aa1-272, clone OTI2H8) LS-C339236
LifeSpan Biosciences
catalog: LS-C339236

mouse monoclonal (OTI2H8)
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CFLAR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
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CFLAR / FLIP Antibody (aa1-272, clone OTI1B1) LS-C339480
LifeSpan Biosciences
catalog: LS-C339480

mouse monoclonal (OTI1B1)
reactivity: human
application: western blot

CFLAR / FLIP Antibody (aa1-480, clone NF6, Biotin) LS-C344788
LifeSpan Biosciences
catalog: LS-C344788

mouse monoclonal (NF6)
reactivity: human
conjugate: biotin
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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CFLAR / FLIP Antibody (aa1-480, clone Dave-2) LS-C344933
LifeSpan Biosciences
catalog: LS-C344933

rat monoclonal (Dave-2)
reactivity: human
application: western blot, immunoprecipitation

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CFLAR / FLIP Antibody (aa1-480, clone NF6) LS-C344945
LifeSpan Biosciences
catalog: LS-C344945

mouse monoclonal (NF6)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

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CFLAR / FLIP Antibody (N-Terminus) LS-C353119
LifeSpan Biosciences
catalog: LS-C353119

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

Western blot analysis of c-FLIP expression in Jurkat (A); SW480 (B); K562 (C) whole cell lysates.
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CFLAR / FLIP Antibody LS-C358696
LifeSpan Biosciences
catalog: LS-C358696

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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CFLAR / FLIP Antibody (aa43-281) LS-C374987
LifeSpan Biosciences
catalog: LS-C374987

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot of CFLAR / FLIP antibody.

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CFLAR / FLIP Antibody LS-C375460
LifeSpan Biosciences
catalog: LS-C375460

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot. All lanes: CFLAR antibody at 1.2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: A431 whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 55 kDa. Observed band size: 55 kDa.

Immunohistochemistry of paraffin-embedded human colon cancer using antibody at dilution of 1:100.
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CFLAR / FLIP Antibody (HRP) LS-C375464
LifeSpan Biosciences
catalog: LS-C375464

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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CFLAR / FLIP Antibody (Biotin) LS-C375465
LifeSpan Biosciences
catalog: LS-C375465

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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CFLAR / FLIP Antibody (FITC) LS-C375466
LifeSpan Biosciences
catalog: LS-C375466

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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CFLAR / FLIP Antibody (150-230 aa, Internal) LS-C382673
LifeSpan Biosciences
catalog: LS-C382673

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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CFLAR / FLIP Antibody (N-Terminal) LS-C386921
LifeSpan Biosciences
catalog: LS-C386921

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of c-FLIP antibody
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