IHC-plus™ ACTN2 Antibody LS-B4217
LifeSpan Biosciences
catalog: LS-B4217
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of methanol-fixed HeLa, using alpha Actinin 2 antibody at 1:200 dilution.

Sample(30 ug whole cell lysate). A: A431. B: H1299. C: HeLa S3. D: Hep G2. 7.5% SDS PAGE. ACTN2 antibody diluted at 1:1000.

Anti-ACTN2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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IHC-plus™ ACTN2 Antibody LS-B4852
LifeSpan Biosciences
catalog: LS-B4852
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescence of paraformaldehyde-fixed HeLa, using actinin, alpha 2 antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate). A: A431. B: H1299. 7.5% SDS PAGE. ACTN2 antibody diluted at 1:1000.

Anti-ACTN2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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ACTN2 Antibody (C-Terminus) LS-C40329
LifeSpan Biosciences
catalog: LS-C40329
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
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ACTN2 Antibody (aa586-635) LS-C31450
LifeSpan Biosciences
catalog: LS-C31450
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
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ACTN2 Antibody (clone 5C5) LS-C95373
LifeSpan Biosciences
catalog: LS-C95373
mouse monoclonal (5C5)
reactivity: human
application: immunohistochemistry
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ACTN2 Antibody (clone EA-53) LS-C95446
LifeSpan Biosciences
catalog: LS-C95446
mouse monoclonal (EA-53)
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
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ACTN2 Antibody (aa751-779) LS-C156006
LifeSpan Biosciences
catalog: LS-C156006
domestic rabbit polyclonal
reactivity: human
application: western blot

ACTN2 Antibody western blot of mouse heart tissue lysates (35 ug/lane). The ACTN2 antibody detected the ACTN2 protein (arrow).
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ACTN2 Antibody (aa751-779, AP) LS-C250767
LifeSpan Biosciences
catalog: LS-C250767
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: AP
application: western blot, ELISA
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ACTN2 Antibody (aa751-779, APC) LS-C250768
LifeSpan Biosciences
catalog: LS-C250768
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: APC
application: western blot, ELISA
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ACTN2 Antibody (aa751-779, Biotin) LS-C250769
LifeSpan Biosciences
catalog: LS-C250769
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA
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ACTN2 Antibody (aa751-779, FITC) LS-C250770
LifeSpan Biosciences
catalog: LS-C250770
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot, ELISA
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ACTN2 Antibody (aa751-779, Azide-free, HRP) LS-C250771
LifeSpan Biosciences
catalog: LS-C250771
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA
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ACTN2 Antibody (aa751-779, PE) LS-C250772
LifeSpan Biosciences
catalog: LS-C250772
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: PE
application: western blot, ELISA
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ACTN2 Antibody (aa1-254) LS-C292610
LifeSpan Biosciences
catalog: LS-C292610
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation


Knockout Varification: Lane 1: Wild-type Hela cell lysate; Lane 2: ACTN2 knockout Hela cell lysate; Predicted MW: 104kDa Observed MW: 104kDa Primary Ab: 1µg/ml Rabbit Anti-Mouse ACTN2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
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ACTN2 Antibody (aa1-254, Biotin) LS-C298543
LifeSpan Biosciences
catalog: LS-C298543
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot


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ACTN2 Antibody (aa1-254, FITC) LS-C301715
LifeSpan Biosciences
catalog: LS-C301715
domestic rabbit polyclonal
reactivity: human, mouse
conjugate: FITC
application: western blot


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ACTN2 Antibody (aa751-779) LS-C319313
LifeSpan Biosciences
catalog: LS-C319313
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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IHC-plus™ ACTN2 Antibody (N-Terminus) LS-B11502
LifeSpan Biosciences
catalog: LS-B11502
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ACTN2 Antibody LS-C332732
LifeSpan Biosciences
catalog: LS-C332732
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of extracts of various cells.
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ACTN2 Antibody (N-Terminus) LS-C351789
LifeSpan Biosciences
catalog: LS-C351789
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of ACTN2 expression in HeLa (A); mouse kidney (B); H9C2 (C) whole cell lysates.

Immunohistochemical analysis of ACTN2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of ACTN2 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ACTN2 Antibody LS-C356811
LifeSpan Biosciences
catalog: LS-C356811
domestic rabbit polyclonal
reactivity: human
application: western blot

All lanes : Anti-ACTN2 Antibody at 1:1000 dilution Lane 1: HeLa whole cell lysates Lane 2: human skeletal muscle lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 104 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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