US01 / p115 Antibody (Internal) LS-C95265
LifeSpan Biosciences
catalog: LS-C95265

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
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IHC-plus™ US01 / p115 Antibody (clone 3F4) LS-B6185
LifeSpan Biosciences
catalog: LS-B6185

mouse monoclonal (3F4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Western blot of USO1 expression in HeLa.

Detection limit for recombinant GST tagged USO1 is 0.3 ng/ml as a capture antibody.

Anti-USO1 / p115 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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US01 / p115 Antibody (aa961-972) LS-C55666
LifeSpan Biosciences
catalog: LS-C55666

domestic goat polyclonal
reactivity: human
application: western blot

Antibody (1 ug/ml) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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US01 / p115 Antibody LS-C136862
LifeSpan Biosciences
catalog: LS-C136862

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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US01 / p115 Antibody (aa679-728) LS-C151763
LifeSpan Biosciences
catalog: LS-C151763

domestic rabbit polyclonal
reactivity: human
application: western blot

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US01 / p115 Antibody (aa70-119) LS-C151764
LifeSpan Biosciences
catalog: LS-C151764

domestic rabbit polyclonal
reactivity: human
application: western blot

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US01 / p115 Antibody (aa932-960) LS-C160464
LifeSpan Biosciences
catalog: LS-C160464

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

USO1 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The USO1 antibody detected the USO1 protein (arrow).

USO1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human pancreas tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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US01 / p115 Antibody (aa932-960, AP) LS-C273145
LifeSpan Biosciences
catalog: LS-C273145

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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US01 / p115 Antibody (aa932-960, APC) LS-C273146
LifeSpan Biosciences
catalog: LS-C273146

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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US01 / p115 Antibody (aa932-960, Biotin) LS-C273147
LifeSpan Biosciences
catalog: LS-C273147

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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US01 / p115 Antibody (aa932-960, FITC) LS-C273148
LifeSpan Biosciences
catalog: LS-C273148

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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US01 / p115 Antibody (aa932-960, Azide-free, HRP) LS-C273149
LifeSpan Biosciences
catalog: LS-C273149

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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US01 / p115 Antibody (aa932-960, PE) LS-C273150
LifeSpan Biosciences
catalog: LS-C273150

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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IHC-plus™ US01 / p115 Antibody (aa913-962) LS-B11369
LifeSpan Biosciences
catalog: LS-B11369

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Anti-US01 / p115 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Western blot analysis of lysates from MCF-7, HeLa, and Jurkat cells, using USO1 Antibody. The lane on the right is blocked with the synthesized peptide.
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US01 / p115 Antibody (aa932-960) LS-C326223
LifeSpan Biosciences
catalog: LS-C326223

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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US01 / p115 Antibody LS-C332251
LifeSpan Biosciences
catalog: LS-C332251

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of extracts of various cell lines.

Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.
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US01 / p115 Antibody LS-C342229
LifeSpan Biosciences
catalog: LS-C342229

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

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US01 / p115 Antibody (890-970 aa, C-terminal) LS-C385257
LifeSpan Biosciences
catalog: LS-C385257

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Western blot of p115 antibody
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