BAF53 / ACTL6A Antibody (clone 3C4) LS-C196606
LifeSpan Biosciences
catalog: LS-C196606
mouse monoclonal (3C4)
reactivity: human
application: western blot, ELISA
citations: 1

Western Blot analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4.Lane 1: ACTL6A transfected lysate(47.5 KDa).Lane 2: Non-transfected lysate.

Detection limit for recombinant GST tagged ACTL6A is approximately 0.3 ng/ml as a capture antibody.
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BAF53 / ACTL6A Antibody (aa35-84) LS-C115969
LifeSpan Biosciences
catalog: LS-C115969
domestic rabbit polyclonal
reactivity: human
application: western blot
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BAF53 / ACTL6A Antibody (aa2-30) LS-C155997
LifeSpan Biosciences
catalog: LS-C155997
domestic rabbit polyclonal
reactivity: human
application: western blot

ACTL6A Antibody western blot of K562 cell line lysates (35 ug/lane). The ACTL6A antibody detected the ACTL6A protein (arrow).
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IHC-plus™ BAF53 / ACTL6A Antibody (aa201-250) LS-B9135
LifeSpan Biosciences
catalog: LS-B9135
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human, Skin: Formalin-Fixed Paraffin-Embedded (FFPE)

Immunofluorescence analysis of HUVEC cells, using ACTL6A Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat and COLO205 cells, using ACTL6A Antibody. The lane on the right is blocked with the synthesized peptide.
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BAF53 / ACTL6A Antibody (Trp237) LS-C191930
LifeSpan Biosciences
catalog: LS-C191930
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry
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BAF53 / ACTL6A Antibody (aa81-130) LS-C201682
LifeSpan Biosciences
catalog: LS-C201682
domestic rabbit polyclonal
reactivity: human
application: western blot
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BAF53 / ACTL6A Antibody (aa2-30, AP) LS-C221359
LifeSpan Biosciences
catalog: LS-C221359
domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA
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BAF53 / ACTL6A Antibody (aa2-30, APC) LS-C221360
LifeSpan Biosciences
catalog: LS-C221360
domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA
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BAF53 / ACTL6A Antibody (aa2-30, Biotin) LS-C221365
LifeSpan Biosciences
catalog: LS-C221365
domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA
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BAF53 / ACTL6A Antibody (aa2-30, FITC) LS-C221366
LifeSpan Biosciences
catalog: LS-C221366
domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA
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BAF53 / ACTL6A Antibody (aa2-30, Azide-free, HRP) LS-C221367
LifeSpan Biosciences
catalog: LS-C221367
domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA
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BAF53 / ACTL6A Antibody (aa2-30, PE) LS-C221368
LifeSpan Biosciences
catalog: LS-C221368
domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA
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BAF53 / ACTL6A Antibody (aa25-75) LS-C286526
LifeSpan Biosciences
catalog: LS-C286526
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
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BAF53 / ACTL6A Antibody (N-Terminus) LS-C305981
LifeSpan Biosciences
catalog: LS-C305981
domestic rabbit polyclonal
reactivity: human
application: western blot
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BAF53 / ACTL6A Antibody LS-C309315
LifeSpan Biosciences
catalog: LS-C309315
domestic rabbit polyclonal
reactivity: human
application: immunoprecipitation
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BAF53 / ACTL6A Antibody (aa2-30) LS-C319307
LifeSpan Biosciences
catalog: LS-C319307
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
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BAF53 / ACTL6A Antibody LS-C334030
LifeSpan Biosciences
catalog: LS-C334030
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, ChIP-Seq

Western blot analysis of extracts of various cell lines.
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BAF53 / ACTL6A Antibody (aa295-330) LS-C344425
LifeSpan Biosciences
catalog: LS-C344425
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of lysates from HeLa, K562, mouse NIH/3T3 cell line and mouse cerebellum tissue lysate (from left to right) with Mouse Actl6a Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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BAF53 / ACTL6A Antibody (aa295-320) LS-C344496
LifeSpan Biosciences
catalog: LS-C344496
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of lysates from K562, HeLa cell line (from left to right) with Actl6a Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
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BAF53 / ACTL6A Antibody (aa361-395) LS-C344638
LifeSpan Biosciences
catalog: LS-C344638
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

Western blot of lysate from mouse ovary tissue lysate with Mouse Actl6a Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.
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BAF53 / ACTL6A Antibody (Internal) LS-C353877
LifeSpan Biosciences
catalog: LS-C353877
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of BAF53A expression in K562 (A); Raw264.7 (B); PC12 (C); H9C2 (D) whole cell lysates.

Immunohistochemical analysis of BAF53A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescent analysis of BAF53A staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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BAF53 / ACTL6A Antibody (aa1-280) LS-C375132
LifeSpan Biosciences
catalog: LS-C375132
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
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