MAPKAPK5 / PRAK Antibody (clone 7H10B4) LS-C41490
LifeSpan Biosciences
catalog: LS-C41490

mouse monoclonal (7H10B4)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

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MAPKAPK5 / PRAK Antibody (phospho-Ser93) LS-C96899
LifeSpan Biosciences
catalog: LS-C96899

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

The anti-Phospho-MAPKAPK5-S93 antibody is used in Western blot to detect Phospho-MAPKAPK5-S93 in mouse heart tissue lysate. PAD4(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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MAPKAPK5 / PRAK Antibody (Thr182) LS-C100742
LifeSpan Biosciences
catalog: LS-C100742

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot of MAPKAPK5 Antibody (T182) in HeLa cell line lysates (35 ug/lane). MAPKAPK5 (arrow) was detected using the purified antibody.
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MAPKAPK5 / PRAK Antibody (aa197-457) LS-C185924
LifeSpan Biosciences
catalog: LS-C185924

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

Sample (30 ug of whole cell lysate). A:293T, B: H1299. 7.5% SDS PAGE. MAPKAPK5 / PRAK antibody diluted at 1:500

Immunofluorescence of paraformaldehyde-fixed HeLa using PRAK antibody at 1:200 dilution.
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MAPKAPK5 / PRAK Antibody (aa148-197) LS-C199228
LifeSpan Biosciences
catalog: LS-C199228

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of lysates from MCF-7 cells, using MAPKAPK5 Antibody. The lane on the right is blocked with the synthesized peptide.
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MAPKAPK5 / PRAK Antibody (phospho-Thr182) LS-C199229
LifeSpan Biosciences
catalog: LS-C199229

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAPKAPK5 (Phospho-Thr182) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3nM 40', using MAPKAPK5 (Phospho-Thr182) Antibody. The lane on the right is blocked with the phospho peptide.
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MAPKAPK5 / PRAK Antibody (aa171-220) LS-C199230
LifeSpan Biosciences
catalog: LS-C199230

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MAPKAPK5 Antibody. The picture on the right is blocked with the synthesized peptide.
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IHC-plus™ MAPKAPK5 / PRAK Antibody (aa306-318) LS-B9567
LifeSpan Biosciences
catalog: LS-B9567

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

MAPKAPK5 antibody and EB11928 (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).

Anti-MAPKAPK5 / PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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IHC-plus™ MAPKAPK5 / PRAK Antibody (aa192-206) LS-B9569
LifeSpan Biosciences
catalog: LS-B9569

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

MAPKAPK5 antibody EB11927 and (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with mouse anti-MK5 / PRAK (and co-precipitation was measured using rabbit anti-ERK3 in the lower panel).

Anti-MAPKAPK5 / PRAK antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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MAPKAPK5 / PRAK Antibody (phospho-Ser93, AP) LS-C279207
LifeSpan Biosciences
catalog: LS-C279207

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (phospho-Ser93, Biotin) LS-C279208
LifeSpan Biosciences
catalog: LS-C279208

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (phospho-Ser93, APC) LS-C279209
LifeSpan Biosciences
catalog: LS-C279209

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (phospho-Ser93, FITC) LS-C279212
LifeSpan Biosciences
catalog: LS-C279212

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (phospho-Ser93, Azide-free, HRP) LS-C279213
LifeSpan Biosciences
catalog: LS-C279213

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (phospho-Ser93, PE) LS-C279214
LifeSpan Biosciences
catalog: LS-C279214

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (Thr182, APC) LS-C279215
LifeSpan Biosciences
catalog: LS-C279215

domestic rabbit polyclonal
reactivity: human
conjugate: APC
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (Thr182, AP) LS-C279216
LifeSpan Biosciences
catalog: LS-C279216

domestic rabbit polyclonal
reactivity: human
conjugate: AP
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (Thr182, Biotin) LS-C279217
LifeSpan Biosciences
catalog: LS-C279217

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (Thr182, FITC) LS-C279218
LifeSpan Biosciences
catalog: LS-C279218

domestic rabbit polyclonal
reactivity: human
conjugate: FITC
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (Thr182, Azide-free, HRP) LS-C279219
LifeSpan Biosciences
catalog: LS-C279219

domestic rabbit polyclonal
reactivity: human
conjugate: HRP
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (Thr182, PE) LS-C279220
LifeSpan Biosciences
catalog: LS-C279220

domestic rabbit polyclonal
reactivity: human
conjugate: PE
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (aa423-473) LS-C289343
LifeSpan Biosciences
catalog: LS-C289343

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

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MAPKAPK5 / PRAK Antibody (aa38-66) LS-C164477
LifeSpan Biosciences
catalog: LS-C164477

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry

Western blot analysis of Mapkapk5 (arrow) using rabbit polyclonal Mouse Mapkapk5 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Mapkapk5 gene.

Mouse Mapkapk5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This demonstrates the use of Mouse Mapkapk5 Antibody (N-term) for immunohistochemistry.
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MAPKAPK5 / PRAK Antibody LS-C308886
LifeSpan Biosciences
catalog: LS-C308886

mouse polyclonal
reactivity: human
application: western blot

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MAPKAPK5 / PRAK Antibody (aa371-471) LS-C308888
LifeSpan Biosciences
catalog: LS-C308888

mouse monoclonal
reactivity: human
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody LS-C310943
LifeSpan Biosciences
catalog: LS-C310943

domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation

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MAPKAPK5 / PRAK Antibody LS-C310946
LifeSpan Biosciences
catalog: LS-C310946

domestic rabbit polyclonal
reactivity: human
application: western blot

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MAPKAPK5 / PRAK Antibody (phospho-Ser93) LS-C327829
LifeSpan Biosciences
catalog: LS-C327829

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (Thr182) LS-C327830
LifeSpan Biosciences
catalog: LS-C327830

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI5E1) LS-C339031
LifeSpan Biosciences
catalog: LS-C339031

mouse monoclonal (OTI5E1)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI1B4) LS-C339063
LifeSpan Biosciences
catalog: LS-C339063

mouse monoclonal (OTI1B4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI1A12) LS-C339091
LifeSpan Biosciences
catalog: LS-C339091

mouse monoclonal (OTI1A12)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI1H3) LS-C339092
LifeSpan Biosciences
catalog: LS-C339092

mouse monoclonal (OTI1H3)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI1D11) LS-C339093
LifeSpan Biosciences
catalog: LS-C339093

mouse monoclonal (OTI1D11)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI4G4) LS-C339094
LifeSpan Biosciences
catalog: LS-C339094

mouse monoclonal (OTI4G4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI1A7) LS-C339095
LifeSpan Biosciences
catalog: LS-C339095

mouse monoclonal (OTI1A7)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI1B3) LS-C339098
LifeSpan Biosciences
catalog: LS-C339098

mouse monoclonal (OTI1B3)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI1F5) LS-C339148
LifeSpan Biosciences
catalog: LS-C339148

mouse monoclonal (OTI1F5)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (aa197-471, clone OTI4H4) LS-C339217
LifeSpan Biosciences
catalog: LS-C339217

mouse monoclonal (OTI4H4)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPKAPK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPKAPK5.
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MAPKAPK5 / PRAK Antibody (120-200 aa) LS-C381421
LifeSpan Biosciences
catalog: LS-C381421

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (140-220 aa, Internal) LS-C385621
LifeSpan Biosciences
catalog: LS-C385621

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry

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MAPKAPK5 / PRAK Antibody (120-200 aa) LS-C385624
LifeSpan Biosciences
catalog: LS-C385624

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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MAPKAPK5 / PRAK Antibody (phospho-Thr182) LS-C415790
LifeSpan Biosciences
catalog: LS-C415790

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Western blot of K562 cell lysate using Phospho-MAPKAPK5(Thr182) Antibody
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