GPR49 / LGR5 Antibody (aa443-473) LS-C98620
LifeSpan Biosciences
catalog: LS-C98620

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western blot of anti-LGR5/GPR49 Antibody in K562 cell line lysates (35 ug/lane). LGR5/GPR49(arrow) was detected using the purified antibody.

Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

LGR5 (GPR49) Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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PathPlus™ GPR49 / LGR5 Antibody (Cytoplasmic Domain) LS-A1236
LifeSpan Biosciences
catalog: LS-A1236

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)

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PathPlus™ GPR49 / LGR5 Antibody (N-Terminus) LS-A1232
LifeSpan Biosciences
catalog: LS-A1232

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry - paraffin section
citations: 1

Human, Pancreas, Islets: Formalin-Fixed Paraffin-Embedded (FFPE)
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PathPlus™ GPR49 / LGR5 Antibody (Cytoplasmic Domain) LS-A1235
LifeSpan Biosciences
catalog: LS-A1235

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section

Human, Brain, neurons: Formalin-Fixed Paraffin-Embedded (FFPE)

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GPR49 / LGR5 Antibody (aa650-700) LS-C143162
LifeSpan Biosciences
catalog: LS-C143162

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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GPR49 / LGR5 Antibody (aa650-700, Biotin) LS-C143163
LifeSpan Biosciences
catalog: LS-C143163

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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GPR49 / LGR5 Antibody (aa650-700, DY650) LS-C143164
LifeSpan Biosciences
catalog: LS-C143164

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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GPR49 / LGR5 Antibody (aa650-700, DY488) LS-C143165
LifeSpan Biosciences
catalog: LS-C143165

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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GPR49 / LGR5 Antibody (aa650-700, DY550) LS-C143167
LifeSpan Biosciences
catalog: LS-C143167

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

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GPR49 / LGR5 Antibody LS-C153478
LifeSpan Biosciences
catalog: LS-C153478

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

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GPR49 / LGR5 Antibody (aa689-719) LS-C165757
LifeSpan Biosciences
catalog: LS-C165757

mouse monoclonal
reactivity: human
application: western blot

LGR5/GPR49 Antibody western blot of K562 cell line lysates (35 ug/lane). The LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).
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GPR49 / LGR5 Antibody (aa250-550, clone OTI1C8) LS-C337372
LifeSpan Biosciences
catalog: LS-C337372

mouse monoclonal (OTI1C8)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.

Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5.
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GPR49 / LGR5 Antibody (aa250-550, clone OTI11B5) LS-C337373
LifeSpan Biosciences
catalog: LS-C337373

mouse monoclonal (OTI11B5)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.

Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5.
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GPR49 / LGR5 Antibody (aa250-550, clone OTI2H7) LS-C337374
LifeSpan Biosciences
catalog: LS-C337374

mouse monoclonal (OTI2H7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (green). Actin filaments were labeled with TRITC-phalloidin (Red), and nuclear with DAPI (Blue).
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GPR49 / LGR5 Antibody (aa250-550, clone OTI8C9) LS-C337376
LifeSpan Biosciences
catalog: LS-C337376

mouse monoclonal (OTI8C9)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.
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GPR49 / LGR5 Antibody (aa250-550, clone OTI7H7) LS-C337377
LifeSpan Biosciences
catalog: LS-C337377

mouse monoclonal (OTI7H7)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.

Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5.
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GPR49 / LGR5 Antibody (aa250-550, clone OTI3E11) LS-C337378
LifeSpan Biosciences
catalog: LS-C337378

mouse monoclonal (OTI3E11)
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.
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GPR49 / LGR5 Antibody (aa250-550, clone OTI14E1) LS-C337418
LifeSpan Biosciences
catalog: LS-C337418

mouse monoclonal (OTI14E1)
reactivity: human
application: western blot, flow cytometry

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.
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GPR49 / LGR5 Antibody (C-Terminus) LS-C340545
LifeSpan Biosciences
catalog: LS-C340545

domestic rabbit polyclonal
reactivity: human
application: western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polyclonal anti-LGR5 antibody at 1:500 dilution.
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